Li Meng, Lu Bin, Chu Li, Zhang Lu, Tao Li-Yuan
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014 Sep;34(9):1130-4.
To observe the effect of Tongxie Yaofang (TY) on the number of mast cells (MCs) and the expression of cytokines in rats with visceral hypersensitivity, and to explore roles of TY in treating visceral hypersensitivity and its possible mechanism.
Totally 30 male adult Sprague Dawley (SD) rats were randomly divided into the blank control group, the model group, and the TY treatment group, 10 in each group. The irritable bowel syndrome (IBS) rat model was established by combining colorectal distention with restraint stress in the TY treatment group and the model group. The visceral hypersensitivity was assessed by abdominal withdrawal reflex (AWR). From the 2nd day of successful modeling, rats in the treatment group were admiministered with TY at the daily dose of 4 g/kg for 4 successive weeks. Equal volume of normal saline was given to rats in the model group for 4 successive weeks. No treatment was given to rats in the blank control group. Four weeks later the number of MCs was counted by using toluidine blue staining. The expression of interleukin-4 (IL-4) and interleukin-9 (IL-9) both in colonic mucosa and serum were measured by enzyme linked immunosorbent assay (ELISA), and the expression of protease-activated receptor type 2 (PAR-2) was detected by Western blot.
Compared with the blank control group, the visceral sensitivity was significantly elevated, the number of MCs in the ileocecal junction increased, and the expression of IL-4, IL-9, and PAR-2 in serum and the colonic mucosa significantly increased (P < 0.05). Compared with the model group, the visceral sensitivity significantly decreased, the number of MCs reduced, and the expression of PAR-2 in the colonic mucosa significantly reduced (all P < 0.05), and the expression of IL-4 in colonic mucosa and IL-9 in serum were obviously reduced in the TY treatment group (P < 0.05).
TY might improve the visceral hypersensitivity by acting on MCs related cytokines and reducing degranulation of MCs.
观察痛泻要方（TY）对内脏高敏感大鼠肥大细胞（MCs）数量及细胞因子表达的影响，探讨TY治疗内脏高敏感性的作用及其可能机制。
将30只成年雄性Sprague Dawley（SD）大鼠随机分为空白对照组、模型组和TY治疗组，每组10只。在TY治疗组和模型组中，采用结直肠扩张联合束缚应激建立肠易激综合征（IBS）大鼠模型。通过腹部撤回反射（AWR）评估内脏高敏感性。从成功建模的第2天起，治疗组大鼠连续4周每天给予4 g/kg的TY。模型组大鼠连续4周给予等量生理盐水。空白对照组大鼠不进行治疗。4周后，用甲苯胺蓝染色法计数MCs数量。采用酶联免疫吸附测定（ELISA）法检测结肠黏膜和血清中白细胞介素-4（IL-4）和白细胞介素-9（IL-9）的表达，并用蛋白质印迹法检测蛋白酶激活受体2（PAR-2）的表达。
与空白对照组相比，内脏敏感性显著升高，回盲部MCs数量增加，血清和结肠黏膜中IL-4、IL-9和PAR-2的表达显著增加（P<0.05）。与模型组相比，内脏敏感性显著降低，MCs数量减少，结肠黏膜中PAR-2的表达显著降低（均P<0.05），TY治疗组结肠黏膜中IL-4的表达和血清中IL-9的表达明显降低（P<0.05）。
TY可能通过作用于与MCs相关的细胞因子并减少MCs的脱颗粒来改善内脏高敏感性。
