Li Bao-Xiu, Li Chun-Yan, Peng Rui-Qing, Wu Xiao-Jun, Wang Hai-Ying, Wan De-Sen, Zhu Xiao-Feng, Zhang Xiao-Shi
State Key Laboratory of Oncology in South China; Biotherapy Center, Sun Yat-sen University, Guangzhou, China.
Autophagy. 2009 Apr;5(3):303-6. doi: 10.4161/auto.5.3.7491. Epub 2009 Apr 26.
Beclin 1 is a key modulator bridging autophagy, apoptosis and differentiation. This study investigated the expression of beclin 1 in human colon cancers and its association with clinicopathological characteristics. A total of 115 cases of cancer tissues with intact follow-up data were obtained from colon cancer patients with stage IIIB. The expression of beclin 1 in cancer nest and adjacent normal tissue was examined with immunohistochemistry. The results showed the immunostaining of beclin 1 was distributed in plasma-membrane, cytoplasm and nucleus in tumor cells, which occurred in 98 cases (85.2%) of the 115 patients. No or modest beclin 1 expression was observed in adjacent noncancerous tissues. The higher level of beclin 1 expression strongly associated with longer survival. Both univariate analysis and multivariate analysis showed that the beclin 1 expression and invasive depth of primary mass (T stage) were independent prognostic factors. Additionally, there was no significant correlation of beclin 1 expression with clinicopathological characteristics, such as sex, age, site of primary mass, pathological classification, grade and invasive depth with the nonparametric correlation Kendall's tau-b test.
Beclin 1是连接自噬、凋亡和分化的关键调节因子。本研究调查了Beclin 1在人类结肠癌中的表达及其与临床病理特征的关系。从IIIB期结肠癌患者中获取了115例具有完整随访数据的癌组织。采用免疫组织化学法检测Beclin 1在癌巢及癌旁正常组织中的表达。结果显示，Beclin 1免疫染色分布于肿瘤细胞的细胞膜、细胞质和细胞核，115例患者中有98例（85.2%）出现这种情况。在癌旁非癌组织中未观察到或仅观察到适度的Beclin 1表达。Beclin 1表达水平越高，生存期越长。单因素分析和多因素分析均显示，Beclin 1表达和原发肿块浸润深度（T分期）是独立的预后因素。此外，通过非参数相关性Kendall's tau-b检验，Beclin 1表达与性别、年龄、原发肿块部位、病理分类、分级和浸润深度等临床病理特征之间无显著相关性。
