从小鼠胚胎成纤维细胞到基质胶I：在小鼠胚胎成纤维细胞存在的情况下传代人胚胎干细胞。
From MEFs to matrigel I: passaging hESCs in the presence of MEFs.
作者信息
Zhang Jin, Khvorostov Ivan, Teitell Michael
机构信息
David Geffen School of Medicine, University of California, Los Angeles, USA.
出版信息
J Vis Exp. 2008 Jun 4(16):722. doi: 10.3791/722.
Abstract
This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells.
摘要
本视频展示了如何在小鼠胚胎成纤维细胞（MEF）饲养层细胞上培养人胚胎干细胞（hESCs）。
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本文引用的文献
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Feeder-free growth of undifferentiated human embryonic stem cells.未分化人胚胎干细胞的无饲养层培养
Nat Biotechnol. 2001 Oct;19(10):971-4. doi: 10.1038/nbt1001-971.
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Embryonic stem cell lines derived from human blastocysts.源自人类囊胚的胚胎干细胞系。
Science. 1998 Nov 6;282(5391):1145-7. doi: 10.1126/science.282.5391.1145.
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