环状DNA的超螺旋密度：通过浮力法测定超螺旋密度的通用方程
Superhelix densities of circular DNA's: a generalized equation for their determination by the bouyant method.
作者信息
Upholt W B
出版信息
Science. 1977 Mar 4;195(4281):891. doi: 10.1126/science.190680.
PMID:190680
Abstract
Equations for the measurement of the supertwisting of circular DNA's from banding positions in buoyant density gradients containing intercalating dyes have required the use of SV40 DNA isolated from virions as a reference DNA. These equations are modified to allow the use of any closed circular DNA of known superhelix density as a reference DNA.
摘要
根据含有嵌入染料的浮力密度梯度中的条带位置来测量环状DNA超螺旋的方程，需要使用从病毒颗粒中分离出的SV40 DNA作为参考DNA。这些方程经过修改，以便能够使用任何已知超螺旋密度的闭环DNA作为参考DNA。
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