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在用于分离闭环DNA的染料浮力密度法中使用溴化乙锭类似物：线粒体DNA超螺旋密度的变化

The use of an ethidium analogue in the dye-buoyant density procedure for the isolation of closed circular DNA: the variation of the superhelix density of mitochondrial DNA.

作者信息

Hudson B, Upholt W B, Devinny J, Vinograd J

出版信息

Proc Natl Acad Sci U S A. 1969 Mar;62(3):813-20. doi: 10.1073/pnas.62.3.813.

DOI:10.1073/pnas.62.3.813
PMID:4308095
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC223671/
Abstract

The separation between open and closed circular DNA in buoyant CsCl gradients containing intercalating dyes depends on the superhelix density of the closed form. These separations are about 1.8 times larger with propidium iodide than with ethidium bromide. The superhelix densities of mitochondrial DNA from HeLa cells and Lytechinus pictus eggs appear to be about two thirds that of mitochondrial DNA from rat and rabbit liver.

摘要

在含有嵌入染料的氯化铯浮力梯度中，开环和闭环环状DNA之间的分离取决于闭环形式的超螺旋密度。与溴化乙锭相比，碘化丙啶导致的这些分离大约大1.8倍。来自HeLa细胞和海胆卵的线粒体DNA的超螺旋密度似乎约为来自大鼠和兔肝脏的线粒体DNA的超螺旋密度的三分之二。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5711/223671/2a3056a4d31d/pnas00105-0198-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5711/223671/2b72bdffc22e/pnas00105-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5711/223671/a7a8b13f10d2/pnas00105-0196-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5711/223671/b767aa0f7471/pnas00105-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5711/223671/94ec675c1d2a/pnas00105-0198-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5711/223671/9a0ce9bca029/pnas00105-0198-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5711/223671/b751a63f7694/pnas00105-0198-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5711/223671/2a3056a4d31d/pnas00105-0198-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5711/223671/2b72bdffc22e/pnas00105-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5711/223671/a7a8b13f10d2/pnas00105-0196-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5711/223671/b767aa0f7471/pnas00105-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5711/223671/94ec675c1d2a/pnas00105-0198-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5711/223671/9a0ce9bca029/pnas00105-0198-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5711/223671/b751a63f7694/pnas00105-0198-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5711/223671/2a3056a4d31d/pnas00105-0198-e.jpg

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1
The use of an ethidium analogue in the dye-buoyant density procedure for the isolation of closed circular DNA: the variation of the superhelix density of mitochondrial DNA.在用于分离闭环DNA的染料浮力密度法中使用溴化乙锭类似物：线粒体DNA超螺旋密度的变化
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Ethidium analogues with improved resolution in the dye-buoyant density procedure.在染料浮力密度法中分辨率得到提高的溴化乙锭类似物。
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本文引用的文献

1
A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.溴化乙锭与核酸之间的荧光复合物。物理化学特性
J Mol Biol. 1967 Jul 14;27(1):87-106. doi: 10.1016/0022-2836(67)90353-1.
2
Studies on circular DNA. II. Number of tertiary turns in papilloma DNA.环状DNA研究。II. 乳头瘤DNA的三级螺旋圈数
J Mol Biol. 1968 Apr 28;33(2):503-5. doi: 10.1016/0022-2836(68)90207-6.
3
Linkage of polynucleotides through phosphodiester bonds by an enzyme from Escherichia coli.来自大肠杆菌的一种酶通过磷酸二酯键连接多核苷酸。
碘化丙啶使荧光显微镜下巴氏杆菌-滑刃线虫感染研究的活体成像成为可能。
Protoplasma. 2021 Mar;258(2):279-287. doi: 10.1007/s00709-020-01567-0. Epub 2020 Oct 17.
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Analyzing live cellularity in the human trabecular meshwork.分析人眼小梁网中的活细胞。
Invest Ophthalmol Vis Sci. 2013 Feb 5;54(2):1039-47. doi: 10.1167/iovs.12-10479.
5
Propidium iodide competes with Ca(2+) to label pectin in pollen tubes and Arabidopsis root hairs.碘化丙啶与 Ca(2+)竞争，标记花粉管和拟南芥根毛中的果胶。
Plant Physiol. 2011 Sep;157(1):175-87. doi: 10.1104/pp.111.182196. Epub 2011 Jul 18.
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Integrated microfluidic devices for combinatorial cell-based assays.用于基于细胞的组合分析的集成微流控装置。
Biomed Microdevices. 2009 Jun;11(3):547-55. doi: 10.1007/s10544-008-9260-x.
7
Early events in phagosome establishment are required for intracellular survival of Legionella pneumophila.吞噬体形成的早期事件是嗜肺军团菌细胞内存活所必需的。
Infect Immun. 1998 Sep;66(9):4450-60. doi: 10.1128/IAI.66.9.4450-4460.1998.
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Inhibition of NF-kappa B activity induces apoptosis in murine hepatocytes.抑制核因子-κB活性可诱导小鼠肝细胞凋亡。
Am J Pathol. 1997 Oct;151(4):891-6.
9
Ethidium bromide-mediated renaturation of denatured closed circular DNAs. The nature of denaturation-resistant fractions of bacteriophage PM2 closed circular DNA.溴化乙锭介导的变性闭环DNA复性。噬菌体PM2闭环DNA抗变性组分的性质。
Nucleic Acids Res. 1980 Feb 11;8(3):673-701. doi: 10.1093/nar/8.3.673.
10
Well-identifiable human chromosomes isolated from mitotic fibroblasts by a new method.通过一种新方法从有丝分裂的成纤维细胞中分离出的易于识别的人类染色体。
Hum Genet. 1982;61(2):157-9. doi: 10.1007/BF00274208.
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The enzymatic repair of DNA. I. Formation of circular lambda-DNA.DNA的酶促修复。I. 环状λ-DNA的形成
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5
Cytoplasmic DNA in the unfertilized sea urchin egg: physical properties of circular mitochondrial DNA and the occurrence of catenated forms.未受精海胆卵中的细胞质DNA：环状线粒体DNA的物理性质及连环体形式的存在
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6
A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.一种用于检测和分离闭环双链DNA的染料浮力密度法：HeLa细胞中的闭环DNA
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Proc Natl Acad Sci U S A. 1967 Nov;58(5):1852-8. doi: 10.1073/pnas.58.5.1852.
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Biochim Biophys Acta. 1968 Apr 22;157(2):429-32. doi: 10.1016/0005-2787(68)90101-9.
10
Early and late helix-coil transitions in closed circular DNA. The number of superhelical turns in polyoma DNA.闭环DNA中的早期和晚期螺旋-线圈转变。多瘤病毒DNA中超螺旋圈数。
J Mol Biol. 1968 Apr 14;33(1):173-97. doi: 10.1016/0022-2836(68)90287-8.