在用于分离闭环DNA的染料浮力密度法中使用溴化乙锭类似物：线粒体DNA超螺旋密度的变化
The use of an ethidium analogue in the dye-buoyant density procedure for the isolation of closed circular DNA: the variation of the superhelix density of mitochondrial DNA.
作者信息
Hudson B, Upholt W B, Devinny J, Vinograd J
出版信息
Proc Natl Acad Sci U S A. 1969 Mar;62(3):813-20. doi: 10.1073/pnas.62.3.813.
Abstract
The separation between open and closed circular DNA in buoyant CsCl gradients containing intercalating dyes depends on the superhelix density of the closed form. These separations are about 1.8 times larger with propidium iodide than with ethidium bromide. The superhelix densities of mitochondrial DNA from HeLa cells and Lytechinus pictus eggs appear to be about two thirds that of mitochondrial DNA from rat and rabbit liver.
摘要
在含有嵌入染料的氯化铯浮力梯度中，开环和闭环环状DNA之间的分离取决于闭环形式的超螺旋密度。与溴化乙锭相比，碘化丙啶导致的这些分离大约大1.8倍。来自HeLa细胞和海胆卵的线粒体DNA的超螺旋密度似乎约为来自大鼠和兔肝脏的线粒体DNA的超螺旋密度的三分之二。
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