St-Pierre Patrick, Hassen I Fekih, Thompson D, Perreault J P
RNA group/Groupe ARN, Département de biochimie, Université de Sherbrooke, Sherbrooke, Québec, Canada.
Virology. 2009 Jan 20;383(2):178-82. doi: 10.1016/j.virol.2008.11.008. Epub 2008 Dec 9.
The peach latent mosaic viroid (PLMVd) is a small, circular RNA species that has been shown to induce RNA silencing in plants. With the goal of better understanding the biological mechanism underlying this process, the siRNAs found in PLMVd infected peach leaves were isolated and sequenced. Analysis of the resulting data prompted several conclusions, including: i. PLMVd strands of both polarities are substrates for the Dicer-like enzymes found in peach leaves; ii. the more highly structured regions of PLMVd trigger the activity of the Dicer-like enzymes; and, iii. the circular PLMVd conformers appear to be favored for transport into the cytoplasm.
桃潜隐花叶类病毒（PLMVd）是一种小型环状RNA，已被证明可在植物中诱导RNA沉默。为了更好地理解这一过程背后的生物学机制，研究人员从感染PLMVd的桃树叶中分离并测序了小干扰RNA（siRNA）。对所得数据的分析得出了几个结论，包括：i. 两种极性的PLMVd链都是桃树叶中发现的Dicer样酶的底物；ii. PLMVd结构更复杂的区域会触发Dicer样酶的活性；iii. 环状PLMVd构象似乎更有利于转运到细胞质中。
