Xing Wei, Zuo Jian-e, Lin Jia, Li Jin
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Huan Jing Ke Xue. 2008 Sep;29(9):2558-63.
The microbial communities in 6 granular sludge samples taken from a lab-scale EGSB reactor operated under different organic loading rates (OLR) at 20 degrees C were studied using PCR-DGGE and RTQ-PCR technique. The results indicate that as the OLR increased from 1.5 kg/(m3 x d) to 10.0 kg/(m3 x d), the microbial communities of archaea and eubacteria in the 6 sludge samples are not changed greatly. The archaea population is highly similar among the 6 samples. The Shannon indexes of archaea population are 5.51, 5.88, 5.47, 5.25, 5.32 and 5.11, respectively. Except the seed sludge, the eubacteria population is similar among the other 5 samples. The Shannon indexes of eubacteria population are 2.97, 5.07, 5.44, 6.38, 6.66 and 5.21, respectively. The dominant archaea in the 6 granular samples are Methanobacterium, Methanocorpusculum, Methanosaeta and Methanospirillum. As the OLR of the reactor increased and the operation time elapsed, Methanocorpusculum parvum and Methanospirillum hungatei, both are hydrotrophic methanogens, become the dominant archaea. The archaea content in the samples decreases slightly at the beginning, but increases later and finally reaches to a level obviously higher than that in the seed sludge.
采用PCR-DGGE和RTQ-PCR技术，对取自实验室规模的膨胀颗粒污泥床（EGSB）反应器、在20℃下不同有机负荷率（OLR）运行的6个颗粒污泥样品中的微生物群落进行了研究。结果表明，随着OLR从1.5 kg/(m³·d)增加到10.0 kg/(m³·d)，6个污泥样品中古菌和真细菌的微生物群落变化不大。6个样品中古菌种群高度相似。古菌种群的香农指数分别为5.51、5.88、5.47、5.25、5.32和5.11。除接种污泥外，其他5个样品中的真细菌种群相似。真细菌种群的香农指数分别为2.97、5.07、5.44、6.38、6.66和5.21。6个颗粒样品中的优势古菌为甲烷杆菌属、甲烷小八叠球菌属、甲烷丝状菌属和甲烷螺菌属。随着反应器OLR的增加和运行时间的推移，嗜氢产甲烷菌小甲烷小八叠球菌和亨氏甲烷螺菌成为优势古菌。样品中的古菌含量开始时略有下降，但随后增加，最终达到明显高于接种污泥的水平。
