Trenkić Milan, Basić Marin, Milentijević Maja, Petrović Aleksandar, Zivković Vesna, Lazarević Viktor
Medical School, Clinic of Gynaecology and Obstetrics, Nis, Srbija.
Vojnosanit Pregl. 2008 Nov;65(11):810-3. doi: 10.2298/vsp0811810t.
BACKGROUND/AIM: Usual histopatological diagnosis of intrauterine pregnancy is made by demonstration of chorionic villi, but in the curettage tissue from intrauterine miscarriage they may not be present in all cases. The use of monoclonal antibody against cytokeratin as a sensitive and reliable marker for the morphologic discrimination between invasive trophoblastic (IT) cells and decidual cells has been well established. The aim of this study was to determine the presence of pregnancy in endometrial curettings when chorionic villi are absent from patients suspected of intrauterine pregnancy.
Twenty cases of endometrial tissue specimens were investigated for cytokeratin and vimentin expression by a double immunostaining for detection of IT cells.
Out of the total number of cases (20) 17 cases expressed cytokeratin 7 positive IT cells, that are an evidence of pregnancy.
The obtained results indicated, that double immunohistochemical demonstration of cytokeratin and vimentin is useful for identifying pregnancy in all chorionic villi-negative cases.
背景/目的：宫内妊娠的常规组织病理学诊断是通过显示绒毛膜绒毛来进行的，但在宫内流产的刮宫组织中，并非所有病例都能见到绒毛。使用抗细胞角蛋白单克隆抗体作为侵袭性滋养层（IT）细胞与蜕膜细胞形态学鉴别敏感且可靠的标志物已得到充分证实。本研究的目的是确定疑似宫内妊娠患者刮宫组织中绒毛膜绒毛缺失时妊娠的存在情况。
对20例子宫内膜组织标本进行细胞角蛋白和波形蛋白表达检测，采用双重免疫染色法检测IT细胞。
在所有病例（20例）中，17例表达细胞角蛋白7阳性IT细胞，这是妊娠的证据。
所得结果表明，细胞角蛋白和波形蛋白的双重免疫组化显示有助于在所有绒毛膜绒毛阴性病例中识别妊娠。
