L'vov D K, Zhchelkanov M Iu, Vlasov N A, Prilipov A G, Deriabin P G, Fediakina I T, Galkina I V, Zaberezhnyĭ A D, Liapina O V, Shliapnikova O V, Kireev D E, Fesenko E E, Kalmykov M V, Vitkova O N, Morozova T N, Proshina E S, Grebennikova T V, Akanina D S, Samokhvalov E I, Al'khovskiĭ S V, Volkov V A, Semenov V I, Gaponov V V, Shmakov N I, Kushnir A T, Kazarian A S, Starikov N S, Petrenko M S, Slavskiĭ A A, Litvin K E, Shcherbakova L O, Frolov A V, Manin T B, Umanets O A, Bandeev V V, Khvan A M, Dunaev V G, Cheledina T P, Abgarian S R, Mikhaĭlovich V M, Zasedatelev A S, Liubchenko E N, Fliagin V N, Tikhonova I F, Maslov D V, Anan'ev V Iu, Baranov N I, Gorelikov V N, Iakovlev S S, Aliper T I, Nepoklonov E A, Suarez D
Vopr Virusol. 2008 Sep-Oct;53(5):4-8.
The epizootic etiologically associated with highly pathogenic avian influenza H5N1 genotype 2.3.2 that is new for Russia among wild and domestic birds in the south of the Primorye Territory during spring migration in April 2008 has been decoded. About 25% of the wild birds of a water complex, which include European teals (Anas crecca), mallard ducks (Anas platyrhynchos), great-crested grebes (Podiceps cristatus), are involved in viral circulation in the area of the Suifun-Khankai plain. Chicken embryos and the cell lines MDCK, SPEV, BHK-21, SW-13 were used to isolate 3 strains from recently deceased hens (A/chicken/Primorje/1/08, A/chicken/Primorje/11/08, and A/chicken/Primorje/12/08) and one strain from a European teal (A/Anas crecca/Primorje/8/08). The strains were deposited in the State Collection of Viruses of the Russian Federation, D. I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences. The nucleotide sequences of the full-sized genomes of A/chicken/Primorje/1/08 and A/Anas crecca/Primorje/8/08 were sent to the International databank GenBank. The strains from domestic and wild birds were shown to be identical. The isolated strains are most close to the strains Alchicken/Viet Nam/10/05, A/chicken/Guangdong/178/04, and A/duck/Viet Nam/12/05. Molecular genetic analysis has indicated that the strains isolated are susceptible to rimantadine and ozeltamivir and less adapted to mammalian cells (particularly, they contain E627 in RV2, which agrees with the biological properties of these strains in vitro). Penetration of the newly isolated virus into the Far East ecosystem provides in the foreseeable future a way for infecting the birds wintering in America and Australia in the nesting places, with further carriage of viral populations there in the period of autumn migrations.
2008年4月春季迁徙期间，俄罗斯滨海边疆区南部野生和家养鸟类中出现了与高致病性H5N1 2.3.2基因型禽流感相关的动物流行病，病因已查明。在绥芬河-汉凯平原地区，约25%的水鸟群落中的野生鸟类，包括欧绿头鸭（Anas crecca）、绿头鸭（Anas platyrhynchos）、凤头鸊鷉（Podiceps cristatus），参与了病毒传播。利用鸡胚以及MDCK、SPEV、BHK-21、SW-13细胞系，从近期死亡的母鸡（A/鸡/滨海边疆区/1/08、A/鸡/滨海边疆区/11/08和A/鸡/滨海边疆区/12/08）中分离出3株病毒，从一只欧绿头鸭（A/Anas crecca/滨海边疆区/8/08）中分离出1株病毒。这些毒株保存在俄罗斯联邦病毒国家保藏中心、俄罗斯医学科学院D. I. 伊万诺夫斯基病毒学研究所。A/鸡/滨海边疆区/1/08和A/Anas crecca/滨海边疆区/8/08全基因组的核苷酸序列已提交至国际数据库GenBank。结果表明，来自家养和野生鸟类的毒株是相同的。分离出的毒株与毒株Alchicken/越南/10/05、A/鸡/广东/178/04和A/鸭/越南/12/05最为接近。分子遗传学分析表明，分离出的毒株对金刚烷胺和奥司他韦敏感，对哺乳动物细胞的适应性较低（特别是，它们在RV2中含有E627，这与这些毒株在体外的生物学特性相符）。新分离出的病毒渗透到远东生态系统中，在可预见的未来，有可能在筑巢地感染在美国和澳大利亚越冬的鸟类，并在秋季迁徙期间将病毒种群传播到那里。
