Osidze D F, Fisenko S A, Khor'kov I A, Nochevnyĭ V T, Sokova V A
Vopr Virusol. 1975 Sep-Oct(5):581-6.
Reproduction of Aueski disease virus in suspension cultures of trysinized cells and mechanically minced tissue of chick, duck and quail embryos was compared. The optimal conditions for cultivation of vaccine and virulent strains of virus in these systems were determined. The advantages and prospects of using suspension cultures of minced avian embryo tissue for preparation of virus materials with high biological activity and in large volumes in comparison with trypsinized cell suspensions were demonstrated.
比较了奥埃斯基病病毒在胰蛋白酶处理的鸡、鸭和鹌鹑胚胎细胞悬浮培养物以及机械切碎组织中的繁殖情况。确定了在这些系统中培养疫苗株和强毒株病毒的最佳条件。证明了与胰蛋白酶处理的细胞悬液相比,使用切碎的禽胚胎组织悬浮培养物制备具有高生物活性的大量病毒材料的优势和前景。