Diez-Orejas Rosalía, Fernández-Arenas Elena
Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain.
Future Microbiol. 2008 Dec;3(6):661-81. doi: 10.2217/17460913.3.6.661.
Candida albicans infection is a significant cause of morbidity and mortality in immunocompromised patients. In vivo and in vitro models have been developed to study both the fungal and the mammalian immune responses. Phagocytic cells (i.e., macrophages) play a key role in innate immunity against C. albicans by capturing, killing and processing the pathogen for presentation to T cells. The use of microarray technology to study global fungal transcriptional changes after interaction with different host cells has revealed how C. albicans adapts to its environment. Proteomic tools complement molecular approaches and computational methods enable the formulation of relevant biological hypotheses. Therefore, the combination of genomics, proteomics and bioinformatics tools (i.e., network analyses) is a powerful strategy to better understand the biological situation of the fungus inside macrophages; part of the fungal population is killed while a significantly high percentage survives.
白色念珠菌感染是免疫功能低下患者发病和死亡的重要原因。已经建立了体内和体外模型来研究真菌和哺乳动物的免疫反应。吞噬细胞（即巨噬细胞）通过捕获、杀死和处理病原体以呈递给T细胞，在针对白色念珠菌的固有免疫中发挥关键作用。利用微阵列技术研究与不同宿主细胞相互作用后真菌的整体转录变化，揭示了白色念珠菌如何适应其环境。蛋白质组学工具补充了分子方法，而计算方法有助于形成相关的生物学假设。因此，基因组学、蛋白质组学和生物信息学工具（即网络分析）的结合是一种强大的策略，可更好地了解巨噬细胞内真菌的生物学状况；部分真菌群体被杀死，而相当高比例的真菌存活下来。
