Tsubura A, Senzaki H, Shikata N, Oishi Y, Morii S
Department of Pathology, Kansai Medical University, Osaka, Japan.
Carcinogenesis. 1991 Aug;12(8):1433-6. doi: 10.1093/carcin/12.8.1433.
The carcinogenic effect of 7,12-dimethylbenz[a]anthracene (DMBA) was examined in the virgin female Japanese house musk shrew, Suncus murinus (family: Soricidae, order: Insectivora). Leukemia, musk gland tumors, pilosebaceous tumors and sarcomas were induced in the DMBA-treated shrews, whereas none of the controls developed any tumors up to 50 weeks of age. DMBA emulsion was administered i.p. at a dose of 1.25 or 2.5 mg once a week, with either four or eight doses being given from 8 weeks of age. Leukemia developed in 100% (9/9), 50% (5/10), 56% (5/9) and 0% (0/10) of the animals treated with a total dose of 20 mg (8 x 2.5 mg), 10 mg (8 x 1.25 mg), 10 mg (4 x 2.5 mg) and 5 mg (4 x 1.25 mg) of DMBA respectively. Leukemia was of the lymphatic and/or mast cell type, and the spleen was the organ invariably involved. A dose-dependent effect of DMBA was not observed for pilosebaceous and musk gland tumors. When 1 mg of DMBA powder was dusted into the subcutaneous tissue at 4 weeks of age, sarcomas developed at the dusted site (69%; 9/13).
在未交配的雌性日本麝鼩（Suncus murinus，科：鼩鼱科，目：食虫目）中检测了7,12-二甲基苯并[a]蒽（DMBA）的致癌作用。经DMBA处理的麝鼩诱发了白血病、麝腺肿瘤、毛囊皮脂腺肿瘤和肉瘤，而对照组在50周龄前均未发生任何肿瘤。DMBA乳剂通过腹腔注射给药，剂量为1.25或2.5 mg，每周一次，从8周龄开始给药4次或8次。接受20 mg（8×2.5 mg）、10 mg（8×1.25 mg）、10 mg（4×2.5 mg）和5 mg（4×1.25 mg）总剂量DMBA处理的动物中，白血病的发生率分别为100%（9/9）、50%（5/10）、56%（5/9）和0%（0/10）。白血病为淋巴和/或肥大细胞类型，脾脏是总是受累的器官。对于毛囊皮脂腺肿瘤和麝腺肿瘤，未观察到DMBA的剂量依赖性效应。在4周龄时将1 mg DMBA粉末撒入皮下组织，撒粉部位发生了肉瘤（69%；9/13）。
