Fujita Y, Oyaizu T, Takahashi H, Oishi Y, Tsubura A
Department of Pathology, Kansai Medical University, Osaka.
Jpn J Cancer Res. 1994 Dec;85(12):1208-13. doi: 10.1111/j.1349-7006.1994.tb02931.x.
The histological changes occurring in the esophageal mucosa of shrews (Suncus murinus) after N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) treatment were investigated sequentially. Six-week-old female shrews were given a 50 micrograms/ml MNNG solution as drinking water for 30 weeks, and 5 selected at random were killed at 10 and 20 weeks of age, and thereafter at 5-week intervals until 45 weeks of age. Controls were killed at 45 weeks of age. The MNNG-induced esophageal lesion in shrews began from basal cell hyperplasia at 20 weeks of age, followed by dysplasia occurring at 25 weeks of age, then progressed toward intraepithelial carcinoma to invasive squamous cell carcinoma at 35 weeks of age. Apparent sequential dysplasia-carcinoma transition was seen. Papillomas were seen from 25 weeks of age but there was no evidence of papilloma-carcinoma sequence. Five MNNG-untreated shrews killed at the end of the experiment were free of esophageal tumors.
对经N-甲基-N'-硝基-N-亚硝基胍（MNNG）处理的麝鼩（Suncus murinus）食管黏膜发生的组织学变化进行了连续研究。六周龄雌性麝鼩饮用含50微克/毫升MNNG溶液的水30周，随机选取5只分别在10周龄和20周龄处死，此后每隔5周处死一批，直至45周龄。对照组在45周龄处死。MNNG诱导的麝鼩食管病变始于20周龄时的基底细胞增生，随后在25周龄时出现发育异常，然后在35周龄时进展为上皮内癌至浸润性鳞状细胞癌。可见明显的发育异常-癌序贯转变。25周龄时可见乳头状瘤，但无乳头状瘤-癌序列的证据。实验结束时处死的5只未经MNNG处理的麝鼩未发现食管肿瘤。
