日本龙虾（Panulirus japonicus）防御素cDNA两种同工型的特征分析
Characterization of two isoforms of Japanese spiny lobster Panulirus japonicus defensin cDNA.
作者信息
Pisuttharachai Duangjai, Yasuike Motoshige, Aono Hideaki, Yano Yutaka, Murakami Keisuke, Kondo Hidehiro, Aoki Takashi, Hirono Ikuo
机构信息
Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.
出版信息
Dev Comp Immunol. 2009 Apr;33(4):434-8. doi: 10.1016/j.dci.2008.11.007. Epub 2008 Dec 13.
Antimicrobial peptides (AMPs) are components of the innate immune responses that form the first line of host defense against pathogens. In this study, cDNAs of two new isoforms of defensin (designated PJD1 and PJD2) from a Japanese spiny lobster Panulirus japonicus haemocytes cDNA library were cloned and sequenced. PJD1 and PJD2 consist of 656 and 673 nucleotides encoding putative proteins of 66 and 64 amino acids, respectively. The isoforms share 74.2% amino acid identity. In a phylogenetic analysis, the peptides clustered with vertebrate defensins and were closely mostly related to chicken beta-defensin. PJD1 and PJD2 were detected in all tissues examined including heart, nerves, intestine, haemocytes, gills and hepatopancreas.
抗菌肽（AMPs）是先天免疫反应的组成部分，构成宿主抵御病原体的第一道防线。在本研究中，从日本龙虾血细胞cDNA文库中克隆并测序了两种新的防御素亚型（命名为PJD1和PJD2）的cDNA。PJD1和PJD2分别由656和673个核苷酸组成，分别编码66和64个氨基酸的假定蛋白质。这些亚型具有74.2%的氨基酸同一性。在系统发育分析中，这些肽与脊椎动物防御素聚类，并且与鸡β-防御素关系最为密切。在包括心脏、神经、肠道、血细胞、鳃和肝胰腺在内的所有检测组织中均检测到PJD1和PJD2。
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