利用亲和层析法从谢氏丙酸杆菌中纯化甲基丙二酰辅酶A变位酶。
Purification of methylmalonyl-CoA mutase from Propionibacterium shermanii using affinity chromatography.
作者信息
Murthy V V, Jones E, Cole T W, Johnson J
出版信息
Biochim Biophys Acta. 1977 Aug 11;483(2):487-91. doi: 10.1016/0005-2744(77)90079-1.
PMID:19074
Abstract
A novel procedure for the purification of methylmalonyl-CA mutase from Propionibacterium shermanii has been described which employs affinity chromatography on a column of immobilized vitamin B-12 linked covalently to Sepharose. The method has the advantage of being simple and rapid, thus enabling the purification of the enzyme to near homogeneity with good yields.
摘要
已描述了一种从谢氏丙酸杆菌中纯化甲基丙二酰辅酶A变位酶的新方法，该方法采用在与琼脂糖共价连接的固定化维生素B-12柱上进行亲和色谱。该方法具有简单快速的优点，从而能够以高收率将酶纯化至接近均一。
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