Marsh N, Leadlay P F, Evans P R
Department of Biochemistry, University of Cambridge, U.K.
J Mol Biol. 1988 Mar 20;200(2):421-2. doi: 10.1016/0022-2836(88)90252-5.
Pink crystals of methylmalonyl-CoA mutase from Propionibacterium shermanii, a coenzyme B12 (5'-deoxyadenosylcobalamin)-dependent enzyme, have been obtained by the hanging-drop method in two different forms. One form lies in the space group P21, with unit cell dimensions a = 122 A, b = 160 A and c = 90 A, with beta = 104 degrees (1 A = 0.1 nm). There are two alpha beta dimers in the asymmetric unit. The crystals diffract to 3.2 A resolution and are suitable for high resolution X-ray diffraction studies.
从谢氏丙酸杆菌中获得了甲基丙二酰辅酶A变位酶的粉红色晶体，该酶是一种依赖辅酶B12（5'-脱氧腺苷钴胺素）的酶，通过悬滴法得到了两种不同形式的晶体。一种形式属于空间群P21，晶胞参数为a = 122 Å，b = 160 Å，c = 90 Å，β = 104°（1 Å = 0.1 nm）。不对称单元中有两个αβ二聚体。这些晶体的衍射分辨率为3.2 Å，适合进行高分辨率X射线衍射研究。
