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大肠杆菌K5荚膜并非在细胞质内的一个受保护区室中合成。

The Escherichia coli K5 capsule is not synthesized in a protected compartment within the cytoplasm.

作者信息

Hudson Thomas, Goldrick Marie, Roberts Ian S

机构信息

University of Manchester, United Kingdom.

出版信息

J Bacteriol. 2009 Mar;191(5):1716-8. doi: 10.1128/JB.01371-08. Epub 2008 Dec 12.

DOI:10.1128/JB.01371-08
PMID:19074385
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2648199/
Abstract

The intracellular expression of the K5 lyase enzyme, which degrades the K5 polysaccharide, decreased cell surface expression of the Escherichia coli K5 capsule. This indicates that biosynthesis of K5 polysaccharide in the cytoplasm is accessible to the action of K5 lyase and is not synthesized within a protected cytoplasmic compartment.

摘要

降解K5多糖的K5裂解酶的细胞内表达降低了大肠杆菌K5荚膜的细胞表面表达。这表明细胞质中K5多糖的生物合成可被K5裂解酶作用，且不是在受保护的细胞质区室中合成的。

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本文引用的文献

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Biosynthesis of the Escherichia coli K1 group 2 polysialic acid capsule occurs within a protected cytoplasmic compartment.大肠杆菌K1组2型聚唾液酸荚膜的生物合成发生在一个受保护的细胞质区室中。
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The sensitivity of escherichia coli strains with K1 surface antigen and rods without this antigen to the bactericidal effect of serum.具有K1表面抗原的大肠杆菌菌株和没有该抗原的杆菌对血清杀菌作用的敏感性。
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Extraintestinal pathogenic Escherichia coli: "the other bad E coli".肠外致病性大肠杆菌：“另一种有害大肠杆菌”
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Identification that KfiA, a protein essential for the biosynthesis of the Escherichia coli K5 capsular polysaccharide, is an alpha -UDP-GlcNAc glycosyltransferase. The formation of a membrane-associated K5 biosynthetic complex requires KfiA, KfiB, and KfiC.鉴定出KfiA是大肠杆菌K5荚膜多糖生物合成所必需的一种蛋白质，它是一种α-UDP-GlcNAc糖基转移酶。膜相关K5生物合成复合物的形成需要KfiA、KfiB和KfiC。
J Biol Chem. 2000 Sep 1;275(35):27311-5. doi: 10.1074/jbc.M004426200.
7
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Mol Microbiol. 1999 Mar;31(5):1307-19. doi: 10.1046/j.1365-2958.1999.01276.x.
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