Eden P A, Blakemore R P
Department of Microbiology, University of New Hampshire, Durham 03824.
Arch Microbiol. 1991;155(5):449-52. doi: 10.1007/BF00244960.
Electroporation methods and conjugal matings were used to transfer several plasmid vectors to Aquaspirillum dispar and Aquaspirillum itersonii. The incompatibility P class plasmid RP4 was conjugally transferred from Escherichia coli HB101 to these spirilla, and the transconjugants subsequently donated the molecule to plasmid-free E. coli and A. dispar strains via conjugal matings. High-voltage electrotransformation was used to transfer plasmids pUCD2, pSa151 and RP4 to A. dispar and A. itersonii, at efficiencies as high as 3 x 10(4) transformants per micrograms plasmid DNA. RP4 DNA isolated from spirillum hosts, but not RP4 from E. coli cells was successfully transferred to A. dispar and A. itersonii by electrotransformation, suggesting that modification and/or restriction activity may be present in these Aquaspirillum species.
采用电穿孔法和接合交配法将几种质粒载体导入不同种的水螺菌（Aquaspirillum dispar）和迭代水螺菌（Aquaspirillum itersonii）。不相容P类质粒RP4通过接合作用从大肠杆菌HB101转移至这些螺菌中，随后这些接合子通过接合交配将该分子传递给无质粒的大肠杆菌和不同种的水螺菌菌株。利用高压电转化法将质粒pUCD2、pSa151和RP4导入不同种的水螺菌和迭代水螺菌，每微克质粒DNA的转化效率高达3×10⁴个转化子。从螺菌宿主中分离得到的RP4 DNA，而非大肠杆菌细胞中的RP4，通过电转化成功导入不同种的水螺菌和迭代水螺菌，这表明这些水螺菌属物种中可能存在修饰和/或限制活性。
