Kilbane J J, Bielaga B A
Institute of Gas Technology, Chicago, IL 60616.
Biotechniques. 1991 Mar;10(3):354-65.
Simplified electroporation methodologies have been developed that reliably yield transformants with only minutes of effort. Neither DNA purification, cells in specific phase of growth, cell washing nor chilled cuvettes are required to obtain transformants. Electroporation can be used to transfer plasmid or chromosomal DNA directly from donor to recipient cells. This simplified direct method of electroporation has been demonstrated to work for both intra- as well as interspecies transformations using a variety of microorganisms. The use of electroporation to purify plasmid DNA was also investigated and found to be inferior to conventional plasmid isolation procedures.
已经开发出简化的电穿孔方法，只需几分钟的操作就能可靠地产生转化体。获得转化体不需要进行DNA纯化、处于特定生长阶段的细胞、细胞洗涤或预冷的比色皿。电穿孔可用于将质粒或染色体DNA直接从供体细胞转移到受体细胞。这种简化的直接电穿孔方法已被证明适用于使用多种微生物进行种内和种间转化。还研究了用电穿孔法纯化质粒DNA，发现其效果不如传统的质粒分离方法。
