Chamley L W, Pattison N S, McKay E J
Department of Obstetrics and Gynaecology, University of Auckland, National Women's Hospital, New Zealand.
Haemostasis. 1991;21(1):25-9. doi: 10.1159/000216198.
Separation of lupus anticoagulant from anticardiolipin antibodies in the serum of a patient containing both antibodies is described. A simple two-step procedure utilizing diethylaminoethylcellulose ion-exchange chromatography followed by Sepharose CL-4B gel filtration chromatography allowed the separation of IgM from IgG isotype. Lupus anticoagulant was found to be exclusively IgM, whilst anticardiolipin antibodies were IgG. This is apparently the first report that anticardiolipin antibodies and lupus anticoagulant can be different isotypes and adds to the increasing evidence that they are separate entities.
本文描述了从一名同时含有狼疮抗凝物和抗心磷脂抗体的患者血清中分离这两种抗体的方法。采用简单的两步法，即先用二乙氨基乙基纤维素离子交换色谱法，再用琼脂糖CL - 4B凝胶过滤色谱法，可将IgM与IgG同种型分离。结果发现狼疮抗凝物仅为IgM型，而抗心磷脂抗体为IgG型。这显然是关于抗心磷脂抗体和狼疮抗凝物可能为不同同种型的首次报道，进一步证明了它们是不同的实体。
