van der Gaast M H, Macklon N S, Beier-Hellwig K, Krusche C A, Fauser B C J M, Beier H M, Classen-Linke I
Department of Obstetrics and Gynaecology, Division of Reproductive Medicine, Erasmus Medical Centre, Rotterdam, The Netherlands.
BJOG. 2009 Jan;116(2):304-12. doi: 10.1111/j.1471-0528.2008.02039.x.
To compare the assessment of endometrial maturation parameters in endometrial secretion samples obtained by a novel minimally invasive technique with those assessed in tissue biopsies.
Prospective study.
University Hospital.
Healthy female volunteers attending a gynaecological outpatient clinic.
Endometrial secretion fluid and tissue sampling 5 days after a spontaneous ovulation assessed with ultrasound.
Progesterone (P) receptor, Ki-67 expression and the Noyes criteria were used to date endometrial biopsies. In the endometrial fluid samples, glycodelin A (GdA), leukaemia inhibitory factor (LIF) and P levels were analysed, and protein content and electrophoresis patterns were determined.
All data were correlated to estradiol (E2) and P serum concentrations. The dating according to histology and immunohistochemical staining patterns correlated significantly with GdA levels (r=0.376, P=0.048) in endometrial fluid samples as well with serum levels of E2 (r=0.568, P=0.001) and P (r=0.408, P=0.023). No correlation was observed between tissue dating and LIF levels and protein content in endometrial fluid samples.
The measurement of GdA in endometrial secretion samples may provide a less invasive method for assessing endometrial maturation in potential conception cycles without disrupting implantation.
比较通过一种新型微创技术获取的子宫内膜分泌样本中子宫内膜成熟参数的评估结果与组织活检中的评估结果。
前瞻性研究。
大学医院。
妇科门诊的健康女性志愿者。
在超声评估自发排卵5天后采集子宫内膜分泌液和组织样本。
使用孕酮（P）受体、Ki-67表达和诺伊斯标准对子宫内膜活检进行日期判定。对子宫内膜液样本分析糖蛋白A（GdA）、白血病抑制因子（LIF）和P水平，并测定蛋白质含量和电泳图谱。
所有数据均与雌二醇（E2）和血清P浓度相关。根据组织学和免疫组化染色模式进行的日期判定与子宫内膜液样本中的GdA水平（r = 0.376，P = 0.048）以及E2（r = 0.568，P = 0.001）和P（r = 0.408，P = 0.023）的血清水平显著相关。在组织日期判定与子宫内膜液样本中的LIF水平和蛋白质含量之间未观察到相关性。
测量子宫内膜分泌样本中的GdA可能为评估潜在受孕周期中的子宫内膜成熟提供一种侵入性较小的方法，且不会干扰着床。
