Department of Pathology, Erlangen University Hospital, Friedrich-Alexander University of Erlangen-Nürnberg, Erlangen, Germany
Kaufbeuren-Ravensburg Institute of Pathology, Ravensburg, Germany.
In Vivo. 2020 Jul-Aug;34(4):1951-1963. doi: 10.21873/invivo.11992.
BACKGROUND/AIM: This study assessed whether a new immunohistochemical dating method allows precise endometrial dating allowing optimal timing for embryo transfer.
A novel method was used for endometrial dating, with parameters including menstrual cycle days, Noyes histological criteria, along with immunohistochemical expression pattern of estrogen and progesterone receptors and proliferation marker Ki-67. Endometrial maturation was analyzed on days +5 to +10 after ovulation or progesterone administration in 217 biopsies from 151 subfertile patients during the secretory phase.
Endometrial maturation varied individually, occurring 1.68±1.67 days late. Comparison of histological maturation with clinical days after ovulation showed a delay of about 2 days.
Endometrial maturation requires 8 days, rather than the expected 6 days, to reach the histological mid-secretory phase. This is not a delay and is also seen in fertile patients. The new analysis method used is superior to that using Noyes criteria alone and provides a better basis for determining conditions for optimal timing of embryo transfers.
背景/目的：本研究评估了一种新的免疫组织化学定龄方法是否能精确预测子宫内膜，从而为胚胎移植选择最佳时机。
我们采用一种新的方法对子宫内膜进行定龄，其参数包括月经周期天数、Noyes 组织学标准以及雌激素和孕激素受体以及增殖标志物 Ki-67 的免疫组织化学表达模式。在 151 例不孕患者的分泌期，对 217 例活检样本在排卵后或孕激素给药后第+5 天至+10 天进行子宫内膜成熟度分析。
子宫内膜成熟度因人而异，平均延迟 1.68±1.67 天。与排卵后临床天数的组织学成熟度比较显示，大约延迟 2 天。
子宫内膜成熟需要 8 天而不是预期的 6 天才能达到组织学的中分泌期。这不是延迟，在生育力正常的患者中也可以看到。使用的新分析方法优于仅使用 Noyes 标准的方法，为确定胚胎移植的最佳时机条件提供了更好的依据。
