Kondoh Masatoshi, Hayashi Atsushi, Okamori Mariko, Motonaga Chie, Enomoto Tadao, Cheng Lei, Shimada Takashi
Central Research Laboratories, Nichinichi Pharmaceutical Corporation Ltd, 518-1417 Mie, Japan.
Br J Nutr. 2009 Jul;102(1):3-7. doi: 10.1017/S0007114508158998. Epub 2008 Dec 16.
The objective of the present study was to assess the effects of Enterococcus sp. strain TN-3 isolated from deep seawater on inhibition of eosinophil accumulation, IgE production and active cutaneous anaphylaxis (ACA). We investigated the effects of viable and non-viable TN-3 on allergen-induced peritoneal eosinophil accumulation in mice. Viable (5.4 x 1010 colony-forming units per 60 mg) or non-viable TN-3 (60 mg) was orally administered to BALB/c mice that had been sensitised with the cedar pollen (Cryptomeria japonica) allergen. Oral administration of non-viable TN-3 was effective in suppressing eosinophil accumulation while viable TN-3 was ineffective. We also examined the dose-response relationship for non-viable TN-3 in regard to eosinophil accumulation, IgE production and ACA in allergen-primed mice. Non-viable TN-3 was orally administered at doses of 15 mg (low dose), 30 mg (medium dose) and 60 mg (high dose) to BALB/c mice that had been sensitised with cedar pollen allergen. The anti-allergic effects expressed as inhibition of eosinophil accumulation, IgE production and ACA were found at the low and high doses, but not at the medium dose. These results suggest that non-viable TN-3 exhibited anti-allergic effects at doses of 15 and 60 mg.
本研究的目的是评估从深层海水中分离出的肠球菌属菌株TN-3对抑制嗜酸性粒细胞积聚、IgE产生和主动皮肤过敏反应（ACA）的影响。我们研究了活的和灭活的TN-3对变应原诱导的小鼠腹腔嗜酸性粒细胞积聚的影响。将活的TN-3（每60毫克含5.4×10¹⁰个菌落形成单位）或灭活的TN-3（60毫克）口服给予已用雪松花粉（日本柳杉）变应原致敏的BALB/c小鼠。口服灭活的TN-3可有效抑制嗜酸性粒细胞积聚，而活的TN-3则无效。我们还研究了灭活的TN-3在变应原致敏小鼠中对嗜酸性粒细胞积聚、IgE产生和ACA的剂量反应关系。将灭活的TN-3以15毫克（低剂量）、30毫克（中剂量）和60毫克（高剂量）的剂量口服给予已用雪松花粉变应原致敏的BALB/c小鼠。在低剂量和高剂量下发现了以抑制嗜酸性粒细胞积聚、IgE产生和ACA表示的抗过敏作用，但在中剂量下未发现。这些结果表明，灭活的TN-3在15毫克和60毫克的剂量下表现出抗过敏作用。
