Duan Lian, Li Yi-Xiong, Hu Guo-Huang, Tang Hui-Huang, Tao Yi-Ming
Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, PR China.
Ai Zheng. 2008 Dec;27(12):1337-40.
BACKGROUND & OBJECTIVE: Ezrin protein is a membrane cytoskeletal crosslinker between the cell membrane and cytoskeleton. Increasing evidence has shown that Ezrin may be associated with tumor invasion and progression. This study was to explore the correlation of Ezrin to metastasis of pancreatic carcinoma.
The expression of Ezrin mRNA in fresh specimens of 32 pancreatic carcinoma, 19 metastatic lesions and 10 non-cancerous pancreatic tissues were assessed by reverse transcription-polymerase chain (RT-PCR).
The mRNA expression of Ezrin was significantly higher in pancreatic carcinoma tissues (1.9+/-1.1) than in non-cancerous tissues (0.9+/-0.5)(P<0.05), significantly higher in metastatic lesions (2.6+/-0.8) than in the primary tumor focus (1.9+/-1.1) (P<0.05), and significantly higher in pancreatic carcinoma lesions with metastasis (2.1+/-1.2) than in those without (1.4+/-0.7) ( P<0.05). Ezrin mRNA was differently expressed in tumors with different clinical pathologic characters. It was highly expressed in poorly differentiated or distantly metastatic carcinomas.
Ezrin gene is closely related to invasion and metastasis of pancreatic carcinoma, which might be used as a marker in predicting the metastasis of pancreatic carcinoma.
埃兹蛋白是细胞膜与细胞骨架之间的一种膜细胞骨架交联蛋白。越来越多的证据表明，埃兹蛋白可能与肿瘤侵袭和进展相关。本研究旨在探讨埃兹蛋白与胰腺癌转移的相关性。
采用逆转录聚合酶链反应（RT-PCR）检测32例胰腺癌新鲜标本、19个转移灶及10例癌旁胰腺组织中埃兹蛋白mRNA的表达。
胰腺癌组织中埃兹蛋白mRNA表达（1.9±1.1）显著高于癌旁组织（0.9±0.5）（P<0.05）；转移灶中埃兹蛋白mRNA表达（2.6±0.8）显著高于原发灶（1.9±1.1）（P<l0.05）；有转移的胰腺癌组织中埃兹蛋白mRNA表达（2.1±1.2）显著高于无转移者（1.4±0.7）（P<0.05）。埃兹蛋白mRNA在不同临床病理特征的肿瘤中表达不同，在低分化或远处转移癌中高表达。
埃兹蛋白基因与胰腺癌的侵袭和转移密切相关，有望作为预测胰腺癌转移的指标。
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