Akisawa N, Nishimori I, Iwamura T, Onishi S, Hollingsworth M A
Eppley Institute for Cancer and Allied Disease, University of Nebraska Medical Center, 600 S. 42nd Street, Omaha, Nebraska 68198-6805, USA.
Biochem Biophys Res Commun. 1999 May 10;258(2):395-400. doi: 10.1006/bbrc.1999.0653.
Ezrin is a membrane cytoskeleton crosslinker protein that is a member of the ERM (ezrin/radixin/moesin) family. Ezrin binds adhesion molecules such as CD43, CD44, ICAM-1, and ICAM-2, which are implicated in cell migration and metastasis. Ezrin is expressed by many tumor cell lines; however, little is known about the function of ezrin in tumorigenesis and metastasis. Here, we investigated expression of ezrin in pancreatic adenocarcinoma cell lines of different metastatic potential. Among 16 pancreatic adenocarcinoma cell lines, several cell lines showed strong expression of ezrin. Two cell lines with high metastatic potential, S2-CP9 and S2-VP10, showed very high levels of ezrin mRNA and protein, whereas other sublines showed lower levels. There was no relationship between the expression levels of ezrin and the differentiation grades of the cell lines. These results suggest that there is a relationship between high expression of ezrin and metastatic potential of pancreatic carcinomas.
埃兹蛋白是一种膜细胞骨架交联蛋白，属于ERM（埃兹蛋白/根蛋白/膜突蛋白）家族。埃兹蛋白可结合诸如CD43、CD44、细胞间黏附分子-1（ICAM-1）和细胞间黏附分子-2（ICAM-2）等黏附分子，这些分子与细胞迁移和转移有关。许多肿瘤细胞系都表达埃兹蛋白；然而，关于埃兹蛋白在肿瘤发生和转移中的功能却知之甚少。在此，我们研究了埃兹蛋白在具有不同转移潜能的胰腺腺癌细胞系中的表达情况。在16种胰腺腺癌细胞系中，有几种细胞系显示出埃兹蛋白的强表达。两种具有高转移潜能的细胞系S2-CP9和S2-VP10，显示出非常高水平的埃兹蛋白信使核糖核酸（mRNA）和蛋白质，而其他亚系则显示出较低水平。埃兹蛋白的表达水平与细胞系的分化程度之间没有关系。这些结果表明，埃兹蛋白的高表达与胰腺癌的转移潜能之间存在关联。
