Wang Xia, Song Yu-Gang, He Ya-Jing, Lin Ying-Zhuo, Wang Xian-Fei, Chen Ye
Research Institute of Gastroenterology of Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Zhonghua Yi Xue Za Zhi. 2008 Sep 23;88(36):2574-7.
To explore the effect of syndecan-1 (Sdc-1) in the pathogenesis of colitis.
Thirty BALB/c mice were forced to drink 4% dextran sodium sulphate (DSS) in distilled water as the sole source of drinking fluid for 7 days, distilled water for 10 days, and 4% DSS in distilled water for another 7 days so as to establish colitis models and then were randomly divided into 3 equal groups: model groups 1, 2, and 3 to be killed 8, 18, and 25 days after the DSS drinking respectively to take their colons. Another 10 mice were fed with distilled water as control group and were killed on Day 8. Microscopy was used to evaluate the histological score of the colon. RT-PCR was used to detect the expression of Sdc-1 mRNA and IL-1 mRNA in the colon mucosa. Immunohistochemistry was conducted to detect the Sdc-1 protein level.
The histological scores of the 3 model groups were all significantly higher than that of the control group (F = 448.717, P < 0.01) and the score was the highest in the model group 1 and then gradually decreased. There was not significant differences in the Sdc-1 mRNA expression among different groups (F = 0.822, P > 0.05). The levels of Sdc-1 protein of the 3 model groups were all significantly lower than that of the control group (F = 865.586, P < 0.01), and the Sdc-1 protein level was the lowest level in the model group1, and then increased gradually. The expression of IL-1 mRNA of the 3 model groups were all significantly higher than that of the control group (F = 103.833, P < 0.01), and the IL-1 mRNA level was the highest in the model group1 and then decreased gradually.
The severity of colitis is associated with the reduction of Sdc-1 protein level, but not with the Sdc-1 mRNA level in the colon mucosa. The reduction of Sdc-1 protein level may be associated to increase of IL-1 level.
探讨syndecan-1（Sdc-1）在结肠炎发病机制中的作用。
30只BALB/c小鼠被迫饮用含4%葡聚糖硫酸钠（DSS）的蒸馏水作为唯一饮用水源7天，再饮用蒸馏水10天，然后再次饮用含4% DSS的蒸馏水7天以建立结肠炎模型，之后随机分为3个相等的组：模型组1、2和3，分别在饮用DSS后8、18和25天处死并取其结肠。另外10只小鼠喂食蒸馏水作为对照组，并在第8天处死。用显微镜评估结肠的组织学评分。用RT-PCR检测结肠黏膜中Sdc-1 mRNA和IL-1 mRNA的表达。进行免疫组织化学检测Sdc-1蛋白水平。
3个模型组的组织学评分均显著高于对照组（F = 448.717，P < 0.01），且评分在模型组1中最高，然后逐渐降低。不同组间Sdc-1 mRNA表达无显著差异（F = 0.822，P > 0.05）。3个模型组的Sdc-1蛋白水平均显著低于对照组（F = 865.586，P < 0.01），且Sdc-1蛋白水平在模型组1中最低，然后逐渐升高。3个模型组的IL-1 mRNA表达均显著高于对照组（F = 103.833，P < 0.01），且IL-1 mRNA水平在模型组1中最高，然后逐渐降低。
结肠炎的严重程度与结肠黏膜中Sdc-1蛋白水平的降低有关，而与Sdc-1 mRNA水平无关。Sdc-1蛋白水平的降低可能与IL-1水平的升高有关。
