白细胞介素-18在小鼠硫酸葡聚糖钠结肠炎中的表达及功能

[Expression and function of interleukin-18 in mouse dextran sulfate sodium colitis].

作者信息

Wen Zhong-Hui, Quyang Qin, Zhang Hu, Yi Zhi-Hui, Wang Yu-Fang

机构信息

Department of Gastroenterology, West China Hospital of Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Sep;36(5):676-8.

PMID:16235535

Abstract

OBJECTIVE

Interleukin-18 (IL-18) is a proinflammatory cytokine that induces Th1 cytokines production and may play a role in the pathogenesis of inflammatory bowel diseases. This study was aimed at detecting the expression and role of IL-18 in colonic mucosa of dextran sulfate sodium (DSS) colitic mice.

METHODS

Expression of IL-18 in colon of DSS colitis was determined by immunohistochemistry. Extract and supernatant of intestinal epithelial cells (IEC) from colon of DSS colitis mice were used to stimulate splenic mononuclear cells (SMC); IFN-gamma and IL-2 concentrations in supernatant of SMC were measured by ELISA. The levels of expression of IL-18 in extract or supernatant of IEC were examined by Western blot.

RESULTS

Colon epithelial cells and part of lamina propria mononuclear cells were stained positive for IL-18 antibody. Stimulation assay and Western blot indicated that IL-18 positive proteins were present in both IEC extract and supernatant.

CONCLUSION

Colon epithelial cells in mice with DSS colitis express and secret pro IL-18 and active IL-18.

摘要

目的

白细胞介素-18（IL-18）是一种促炎细胞因子，可诱导Th1细胞因子的产生，并可能在炎症性肠病的发病机制中起作用。本研究旨在检测IL-18在葡聚糖硫酸钠（DSS）结肠炎小鼠结肠黏膜中的表达及作用。

方法

采用免疫组织化学法检测DSS结肠炎小鼠结肠中IL-18的表达。用DSS结肠炎小鼠结肠的肠上皮细胞（IEC）提取物和上清液刺激脾单核细胞（SMC）；采用酶联免疫吸附测定法（ELISA）检测SMC上清液中γ干扰素（IFN-γ）和白细胞介素-2（IL-2）的浓度。通过蛋白质印迹法检测IEC提取物或上清液中IL-18的表达水平。