Henss J, Bodemer W, Batsford S R, Vogt A
Z Immunitatsforsch Immunobiol. 1977 Feb;152(5):381-7.
Following immunization of mice with SRBC the kinetics of antibody synthesis by individual immunocompetent cells during the primary immune response were investigated. The diameter of the average hemolytic plaque using a modified Jerne plaquing technique, was taken to be an estimate of the antibody production by a single cell. We found an increase, followed by a decrease, of the average plaque diameter during the primary immune response. The increase of average plaque diameters is probably due to the raised rate of synthesis of antibody molecules. This increase can be accelerated by adjuvants. The decrease late in the primary immune response could be explained by an internally regulated switch from IgM to IgG antibody synthesis. The results are consistent with the mathematical approach of HELLWIG (1,2).
用绵羊红细胞免疫小鼠后，研究了初次免疫应答期间单个免疫活性细胞抗体合成的动力学。采用改良的耶尔恩斑块技术，将平均溶血斑的直径作为单个细胞抗体产生的估计值。我们发现在初次免疫应答期间，平均斑块直径先增加后减小。平均斑块直径的增加可能是由于抗体分子合成速率提高所致。佐剂可加速这种增加。初次免疫应答后期的减小可通过从IgM到IgG抗体合成的内部调节转换来解释。这些结果与HELLWIG的数学方法一致(1,2)。
