Garrido V, Torroba L, García-Jalón I, Vitas A I
Department of Microbiology and Parasitology, University of Navarre, Pamplona, Spain.
Euro Surveill. 2008 Dec 4;13(49):19058.
We monitored the incidence of human listeriosis in Navarre, a region in north of Spain between 1995 and 2005, and carried out the characterisation of Listeria monocytogenes isolates obtained from clinical samples and ready-to-eat products (sliced cooked meat, smoked salmon and liver pate). The active surveillance requesting hospitals to notify all listeriosis cases (n=40) yielded higher incidence rates (average annual rate 0.65/100,000 inhabitants, range 0.18-1.18/100,000 inhabitants) than expected. Pregnant women were the largest group affected (n=13, 32.5% of the cases), with a peak in incidence during the last three years of the study period. From the 40 human cases we obtained 33 Listeria isolates. Serological and molecular characterisation by PFGE identified 20 different pulsotypes, which on three occasions enabled us to link sporadic cases into clusters. Although we could not identify the incriminated food product we found two clinical pulsotypes among smoked salmon and cooked meat isolates. Surveillance of listeriosis in Spain should be improved and coordinated with other European Union Member States in order to better estimate the burden of disease and to prevent foodborne outbreaks.
我们监测了1995年至2005年间西班牙北部纳瓦拉地区人类李斯特菌病的发病率，并对从临床样本和即食产品（切片熟肉、烟熏三文鱼和肝酱）中分离出的单核细胞增生李斯特菌进行了特征分析。主动监测要求医院报告所有李斯特菌病病例（n = 40），其发病率（年均发病率为0.65/10万居民，范围为0.18 - 1.18/10万居民）高于预期。孕妇是受影响最大的群体（n = 13，占病例的32.5%），在研究期的最后三年发病率达到峰值。从40例人类病例中我们获得了33株李斯特菌分离株。通过脉冲场凝胶电泳（PFGE）进行的血清学和分子特征分析确定了20种不同的脉冲型，有三次我们能够将散发病例关联成簇。尽管我们无法确定致病食品，但我们在烟熏三文鱼和熟肉分离株中发现了两种临床脉冲型。西班牙的李斯特菌病监测应加以改进，并与其他欧盟成员国进行协调，以便更好地估计疾病负担并预防食源性疾病暴发。
