Zhu Chengliang, Zhang Rui, Liu Li, Rasool Sahibzada T, Mu Yongxin, Sun Wei, Hao Qian, Liu Fang, Zhu Ying, Wu Jianguo
The State Key Laboratory of Virology and College of Life Sciences, Chinese-French Liver Disease Research Institute (Zhongnan Hospital), Wuhan University, Wuhan, PR China.
Clin Immunol. 2009 Apr;131(1):92-7. doi: 10.1016/j.clim.2008.10.011. Epub 2008 Dec 9.
The immune system plays important roles in determining the outcomes of hepatitis B virus (HBV) infection. Interleukin-27 (IL-27) is a recently identified pro-inflammatory cytokine produced by macrophages, dendritic cells, and epithelial cells. To determine the correlation between HBV infection and IL-27 expression, we investigated the serum IL-27 levels in patients with HBV infection and in healthy individuals. Results showed that IL-27 was significantly elevated in patients as compared to healthy individuals (P<0.001). IL-27 was also detected at higher levels in patients with liver cirrhosis or hepatocellular carcinoma than those with acute hepatitis B or chronic hepatitis B (P<0.05). We also found that IL-27 expression was influenced by HBV e antigen. In addition, our in vitro studies demonstrated that IL-27 promoter activity, mRNA and protein expression were all stimulated in cells transfected with infectious HBV clone.
免疫系统在决定乙型肝炎病毒（HBV）感染的结果中发挥着重要作用。白细胞介素-27（IL-27）是一种最近发现的由巨噬细胞、树突状细胞和上皮细胞产生的促炎细胞因子。为了确定HBV感染与IL-27表达之间的相关性，我们调查了HBV感染患者和健康个体的血清IL-27水平。结果显示，与健康个体相比，患者体内的IL-27显著升高（P<0.001）。肝硬化或肝细胞癌患者体内的IL-27水平也高于急性乙型肝炎或慢性乙型肝炎患者（P<0.05）。我们还发现IL-27的表达受HBV e抗原影响。此外，我们的体外研究表明，用传染性HBV克隆转染的细胞中，IL-27启动子活性、mRNA和蛋白表达均受到刺激。
