Horváth Rudolf, Budinský Vít, Kayserová Jana, Kalina Tomás, Formánková Renata, Starý Jan, Bartůnková Jirina, Sedlácek Petr, Spísek Radek
Institute of Immunology, Charles University, 2nd Faculty of Medicine, University Hospital Motol, Prague, Czech Republic.
Clin Immunol. 2009 Apr;131(1):60-9. doi: 10.1016/j.clim.2008.10.009. Epub 2008 Dec 9.
Allogeneic hematopoetic stem cell transplantation (HSCT) represents a unique opportunity to monitor the kinetics of reconstitution of dendritic cells (DCs) and their dynamics in distinct pathologies. We analyzed DCs reconstitution after myeloablative HSCT. We separately analyzed patients with acute GVHD. DCs were monitored from the earliest phase of hematopoetic reconstitution until day +365. Both myeloid DCs and plasmacytoid DCs appeared at earliest stages after engraftment and relative numbers within white blood cells compartment peaked between days 19-25 after HSCT. Their proportion then gradually declined and absolute numbers of both DC subsets remained lower than in controls during the whole follow-up. Patients with acute GVHD had significantly lower numbers of circulating DCs. Decrease in DC counts preceded onset of clinical symptoms by at least 24 h and was independent of corticosteroids administration. This study reveals quantification of plasmacytoid and myeloid DCs as a potential biomarker for the prediction of acute GVHD development.
异基因造血干细胞移植（HSCT）为监测树突状细胞（DCs）的重建动力学及其在不同病理状态下的动态变化提供了独特的契机。我们分析了清髓性HSCT后DCs的重建情况。我们分别分析了急性移植物抗宿主病（GVHD）患者。从造血重建的最早阶段直至+365天对DCs进行监测。髓样DCs和浆细胞样DCs在植入后的最早阶段均出现，且在HSCT后第19 - 25天，白细胞区室中的相对数量达到峰值。随后其比例逐渐下降，在整个随访期间，两个DC亚群的绝对数量均低于对照组。急性GVHD患者循环DCs的数量显著更低。DC计数的下降比临床症状出现至少提前24小时，且与皮质类固醇的使用无关。本研究揭示，浆细胞样和髓样DCs的定量分析可作为预测急性GVHD发生的潜在生物标志物。
