Ying Zuo-Lin, Li Xiao-Jie, Dang Hong, Wang Feng, Xu Xiao-Yan
Department of Dermatology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
Experimental Research Center, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, P.R. China.
Exp Ther Med. 2014 May;7(5):1354-1358. doi: 10.3892/etm.2014.1568. Epub 2014 Feb 20.
Saikosaponin-d (Ssd) is a triterpenoid saponin derived from L., which has been shown to exhibit a variety of pharmacological properties, including anti-inflammatory, antibacterial and antiviral properties. The aim of the present study was to investigate the effect of Ssd on the differentiation, maturation and function of human monocyte-derived dendritic cells (DCs) isolated from condylomata acuminata patients. The results of the present study demonstrated that Ssd reduced the differentiation of DCs, as evidenced by decreased expression levels of cluster of differentiation (CD)1a, CD80 and CD86 molecules and increased CD14 expression. Expression levels of the mannose receptor and CD32 were also significantly elevated, which was associated with enhanced fluorescein isothiocyanate-dextran endocytic activity. Furthermore, Ssd treatment promoted DC maturation by increasing the expression levels of CD40, CD83, CD80 and CD86. In addition, the function of mature DCs, including the secretion of IL-12 and the stimulation of lymphocyte proliferation, was significantly increased following Ssd administration. In conclusion, the present study indicated that Ssd exhibited immunomodulatory effects and may be a novel potent chemopreventive drug candidate for the treatment of condylomata acuminata.
柴胡皂苷-d（Ssd）是一种从柴胡中提取的三萜皂苷，已被证明具有多种药理特性，包括抗炎、抗菌和抗病毒特性。本研究的目的是探讨Ssd对从尖锐湿疣患者分离的人单核细胞衍生树突状细胞（DCs）的分化、成熟和功能的影响。本研究结果表明，Ssd降低了DCs的分化，表现为分化簇（CD）1a、CD80和CD86分子表达水平降低以及CD14表达增加。甘露糖受体和CD32的表达水平也显著升高，这与异硫氰酸荧光素-葡聚糖内吞活性增强有关。此外，Ssd处理通过增加CD40、CD83、CD80和CD86的表达水平促进DC成熟。此外，给予Ssd后，成熟DCs的功能，包括IL-12的分泌和淋巴细胞增殖刺激，显著增强。总之，本研究表明Ssd具有免疫调节作用，可能是一种治疗尖锐湿疣的新型有效化学预防药物候选物。
