Dzikiewicz-Krawczyk Agnieszka, Piontek Paulina, Szweykowska-Kulińska Zofia, Jarmołowski Artur
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland.
Acta Biochim Pol. 2008;55(4):825-8. Epub 2008 Dec 16.
In this study we investigated whether in plants, like in mammals, components of the nuclear cap-binding protein complex (CBC) are involved in nonsense-mediated mRNA decay (NMD). We selected several genes producing at least two alternatively spliced mRNA variants: one with a premature termination codon (PTC+) and another without it (PTC-). For each gene the PTC+/PTC- ratio was calculated using RT-PCR and direct sequencing in four Arabidopsis thaliana lines: wild type, the NMD mutant atupf3-1 and two CBC mutants: cbp20 and abh1. Whereas in the NMD mutant the ratios of PTC+/PTC- splice variants were higher than in wild-type plants, the two CBC mutants investigated showed no change in the PTC+/PTC- ratios. Our results suggest that neither CBP20 nor CBP80 is involved in NMD in A. thaliana.
在本研究中，我们调查了在植物中，是否像在哺乳动物中一样，核帽结合蛋白复合体（CBC）的组分参与无义介导的mRNA降解（NMD）。我们选择了几个产生至少两种可变剪接mRNA变体的基因：一种带有提前终止密码子（PTC+），另一种没有（PTC-）。对于每个基因，使用RT-PCR和直接测序法在四个拟南芥品系中计算PTC+/PTC-比率：野生型、NMD突变体atupf3-1和两个CBC突变体：cbp20和abh1。虽然在NMD突变体中，PTC+/PTC-剪接变体的比率高于野生型植物，但所研究的两个CBC突变体的PTC+/PTC-比率没有变化。我们的结果表明，CBP20和CBP80均不参与拟南芥的NMD。
