来自绿色链霉菌ATCC 21892的博来霉素家族抗肿瘤抗生素佐尔巴霉素的生物合成基因簇。
The biosynthetic gene cluster of zorbamycin, a member of the bleomycin family of antitumor antibiotics, from Streptomyces flavoviridis ATCC 21892.
作者信息
Galm Ute, Wendt-Pienkowski Evelyn, Wang Liyan, George Nicholas P, Oh Tae-Jin, Yi Fan, Tao Meifeng, Coughlin Jane M, Shen Ben
机构信息
Division of Pharmaceutical Sciences, Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin-Madison, 777 Highland Ave., Madison, Wisconsin 53705, USA.
出版信息
Mol Biosyst. 2009 Jan;5(1):77-90. doi: 10.1039/b814075h. Epub 2008 Nov 12.
Abstract
The biosynthetic gene cluster for the glycopeptide-derived antitumor antibiotic zorbamycin (ZBM) was cloned by screening a cosmid library of Streptomyces flavoviridis ATCC 21892. Sequence analysis revealed 40 ORFs belonging to the ZBM biosynthetic gene cluster. However, only 23 and 22 ORFs showed striking similarities to the biosynthetic gene clusters for the bleomycins (BLMs) and tallysomycins (TLMs), respectively; the remaining ORFs do not show significant homology to ORFs from the related BLM and TLM clusters. The ZBM gene cluster consists of 16 nonribosomal peptide synthetase (NRPS) genes encoding eight complete NRPS modules, three incomplete didomain NRPS modules, and eight freestanding single NRPS domains or associated enzymes, a polyketide synthase (PKS) gene encoding one PKS module, six sugar biosynthesis genes, as well as genes encoding other biosynthesis and resistance proteins. A genetic system using Escherichia coli-Streptomyces flavoviridis intergeneric conjugation was developed to enable ZBM gene cluster boundary determinations and biosynthetic pathway manipulations.
摘要
通过筛选黄变链霉菌ATCC 21892的黏粒文库，克隆了糖肽类抗肿瘤抗生素佐尔博霉素（ZBM）的生物合成基因簇。序列分析揭示了属于ZBM生物合成基因簇的40个开放阅读框（ORF）。然而，分别只有23个和22个ORF与博来霉素（BLM）和塔里霉素（TLM）的生物合成基因簇有显著相似性；其余的ORF与相关BLM和TLM基因簇中的ORF没有明显的同源性。ZBM基因簇由16个非核糖体肽合成酶（NRPS）基因组成，这些基因编码8个完整的NRPS模块、3个不完整的双结构域NRPS模块以及8个独立的单个NRPS结构域或相关酶，1个编码1个聚酮合酶（PKS）模块的聚酮合酶基因，6个糖生物合成基因，以及编码其他生物合成和抗性蛋白的基因。开发了一种利用大肠杆菌-黄变链霉菌属间接合的遗传系统，用于确定ZBM基因簇的边界和操纵生物合成途径。
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