Cutler L S, Christian C P, Rendell J K
Department of Oral Diagnosis, School of Dental Medicine, University of Connecticut Health Center, Farmington 06032.
Arch Oral Biol. 1991;36(5):389-95. doi: 10.1016/0003-9969(91)90010-R.
This study examined the profile of S-GAGs synthesized by presecretory and secretory units isolated from rats at 17, 18 and 21 days in utero and 1, 6 and 35 days after birth. The units were incubated for 2 h in medium containing [35S]-sodium sulphate and then processed and analysed. Secretory units from 17-day embryonic presecretory units produced a S-GAG profile composed of approx. 73% chondroitin sulphate and 26% heparan sulphate. When cells of the embryonic units undergo cytodifferentiation to become secretory cells (18 days in utero), there is a major change in the relative amounts of S-GAG synthesized with 54% of the S-GAG produced being heparan sulphate and 41% chondroitin sulphate. There is a progressive increase in the relative amount of heparan sulphate produced and a concomitant decline in chondroitin sulphate as the secretory compartment of the gland matures. By 35 days after birth, the secretory units produced a S-GAG profile that was greater than 85% heparan sulphate and less than 10% chondroitin sulphate. The ratio of heparan sulphate/chondroitin sulphate production was 0.36 by 17-day embryonic presecretory units and shifted to 9.1 by 35-day postnatal units.
本研究检测了从子宫内17、18和21天以及出生后1、6和35天的大鼠分离出的前分泌单位和分泌单位合成的硫酸化糖胺聚糖（S-GAGs）的概况。将这些单位在含有[35S] - 硫酸钠的培养基中孵育2小时，然后进行处理和分析。来自17天胚胎前分泌单位的分泌单位产生的S-GAG概况约由73%的硫酸软骨素和26%的硫酸乙酰肝素组成。当胚胎单位的细胞进行细胞分化成为分泌细胞时（子宫内18天），合成的S-GAG的相对量发生了重大变化，所产生的S-GAG中有54%是硫酸乙酰肝素，41%是硫酸软骨素。随着腺体分泌区的成熟，硫酸乙酰肝素产生的相对量逐渐增加，而硫酸软骨素则随之减少。到出生后35天，分泌单位产生的S-GAG概况中硫酸乙酰肝素含量超过85%，硫酸软骨素含量低于10%。17天胚胎前分泌单位硫酸乙酰肝素/硫酸软骨素的产生比例为0.36，到出生后35天的单位则转变为9.1。
