Unnikrishnan V S, Sudhakaran P R
Indian J Biochem Biophys. 1989 Dec;26(6):377-80.
Administration of (D+) catechin (100 mg/kg body wt) to rats resulted in an increase in the amount of total sulphated glycosaminoglycans (GAG) in liver. The increase was more pronounced in the case of heparan sulphate than chondroitin sulphate and dermatan sulphate. The liver slices prepared from catechin-treated rats showed a significant increase in the rate of incorporation of 35S-sulphate into GAG. Similarly there was a concentration-dependent increase in the rate of 35S-sulphate incorporation into GAG by normal liver slices in presence of catechin in vitro. Susceptibility to nitrous acid degradation and chondroitinase ABC digestion showed that more than 80% of the GAG labelled in vivo with 35S-sulphate, was heparan sulphate and about 10% chondroitin sulphate and dermatan sulphate. Gel filtration of the 35S-labelled material isolated from livers of normal and catechin-treated animals over sephacryl S-300 did not show any difference probably excluding the possibility of free GAG chains initiated on catechin or any of its metabolites in vivo. These results indicate that catechin stimulates the synthesis of sulphated GAG, particularly heparan sulphate in liver.
给大鼠注射（D+）儿茶素（100毫克/千克体重）会导致肝脏中总硫酸化糖胺聚糖（GAG）含量增加。硫酸乙酰肝素的增加比硫酸软骨素和硫酸皮肤素更为明显。用儿茶素处理的大鼠制备的肝切片显示，35S-硫酸盐掺入GAG的速率显著增加。同样，在体外存在儿茶素的情况下，正常肝切片中35S-硫酸盐掺入GAG的速率也呈浓度依赖性增加。对亚硝酸降解和软骨素酶ABC消化的敏感性表明，体内用35S-硫酸盐标记的GAG中，超过80%是硫酸乙酰肝素，约10%是硫酸软骨素和硫酸皮肤素。对从正常和儿茶素处理动物的肝脏中分离出的35S标记物质在Sephacryl S-300上进行凝胶过滤，未显示出任何差异，这可能排除了体内儿茶素或其任何代谢产物引发游离GAG链的可能性。这些结果表明，儿茶素刺激肝脏中硫酸化GAG的合成，尤其是硫酸乙酰肝素。
