Kitagawa H, Nakada H, Fukui S, Kawasaki T, Yamashina I
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Biochem Biophys Res Commun. 1991 Aug 15;178(3):1429-36. doi: 10.1016/0006-291x(91)91053-f.
Oligosaccharides with the sialyl-Le(a) structure have been isolated on an affinity column of a monoclonal antibody, MSW 113, from mucin-type glycoproteins derived from the surfaces of SW 1116 and LS 180 cells, and their secretions. The oligosaccharides were polydisperse with respect to molecular size, the oligosaccharides derived from glycoproteins in culture media being larger than those in cell lysates, as assessed by gel filtration. Some of the oligosaccharides were susceptible to degradation by endo-beta-galactosidase (E. freundii), as judged from the change in the gel filtration pattern. These results indicate that oligosaccharides with the sialyl-Le(a) structure derived from mucin-type glycoproteins produced by human colonic cancer cells are extremely large in size and complex in structure, and that some of them contain the poly-N-acetyllactosamine structure.
具有唾液酸化-Le(a)结构的寡糖已通过单克隆抗体MSW 113的亲和柱从源自SW 1116和LS 180细胞表面及其分泌物的粘蛋白型糖蛋白中分离出来。通过凝胶过滤评估，这些寡糖在分子大小方面具有多分散性，培养基中糖蛋白衍生的寡糖比细胞裂解物中的寡糖更大。从凝胶过滤模式的变化判断，一些寡糖易被内切β-半乳糖苷酶（弗氏埃希菌）降解。这些结果表明，人结肠癌细胞产生的粘蛋白型糖蛋白衍生的具有唾液酸化-Le(a)结构的寡糖在大小上极大且结构复杂，并且其中一些含有多聚N-乙酰乳糖胺结构。
