从人乳中免疫亲和分离唾液酸化-Le(a)寡糖。

Immunoaffinity isolation of a sialyl-Le(a) oligosaccharide from human milk.

作者信息

Kitagawa H, Nakada H, Numata Y, Kurosaka A, Fukui S, Funakoshi I, Kawasaki T, Shimada K, Inagaki F, Yamashina I

机构信息

Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University.

出版信息

J Biochem. 1988 Oct;104(4):591-4. doi: 10.1093/oxfordjournals.jbchem.a122516.

DOI:10.1093/oxfordjournals.jbchem.a122516

PMID:3071532

Abstract

A cancer-associated antigen, sialyl-Le(a) oligosaccharide, was isolated from human milk using a monoclonal antibody recognizing carbohydrate moieties of mucin-type glycoproteins. The structure was identified as: (Formula: see text) based on 500-MHz 1H-NMR spectroscopy. This oligosaccharide comprises 0.07% of sialyloligosaccharides in human milk. The NMR spectra of two fellow oligosaccharides, Le(a) oligosaccharide (or lacto-N-fucopentaose II) and LS-tetrasaccharide a, are also given.

摘要

一种与癌症相关的抗原，唾液酸化Le(a)寡糖，使用识别粘蛋白型糖蛋白碳水化合物部分的单克隆抗体从人乳中分离出来。基于500兆赫的1H核磁共振光谱，其结构被鉴定为：（分子式：见正文）。这种寡糖占人乳中唾液酸化寡糖的0.07%。还给出了另外两种寡糖，Le(a)寡糖（或乳糖-N-岩藻五糖II）和LS-四糖a的核磁共振光谱。