Tabchoury Cínthia Pereira Machado, Sousa Maria Clara K, Arthur Rodrigo Alex, Mattos-Graner Renata Oliveira, Del Bel Cury Altair Antoninha, Cury Jaime Aparecido
Department of Physiological Science, Dental School of Piracicaba, State University of Campinas, Piracicaba, SP, Brazil.
J Appl Oral Sci. 2008 Nov-Dec;16(6):403-7. doi: 10.1590/s1678-77572008000600009.
Streptococcus mutans has been considered one of the main etiological agents of dental caries and the genotypic diversity rather than its salivary counts may be considered as a virulence factor of this bacterium. For genotyping with polymerase chain reaction (PCR) with arbitrary primers, several primers have been used in order to improve complexity and specificity of amplicon patterns. Thus, the aim of this study was to evaluate the degree of agreement of genotypic identification among AP-PCR reactions performed with 5 distinct arbitrary primers of S. mutans isolated from saliva. Stimulated saliva was collected from 11 adult volunteers for isolation of S. mutans, and a total of 88 isolates were genotyped with arbitrary primers OPA 02, 03, 05, 13 and 18. Fourteen distinct genotypes were identified in the saliva samples. Most volunteers (9 out of 11) presented only one genotype. The results of the present study suggest that primers OPA 02, 03, 05 and 13 were suitable for genotypic identification of S. mutans isolates of saliva from adult volunteers.
变形链球菌被认为是龋齿的主要病因之一，该细菌的基因型多样性而非其唾液计数可被视为一种毒力因子。为了使用任意引物通过聚合酶链反应（PCR）进行基因分型，人们使用了多种引物以提高扩增子模式的复杂性和特异性。因此，本研究的目的是评估用从唾液中分离出的5种不同的变形链球菌任意引物进行的AP-PCR反应之间基因型鉴定的一致程度。从11名成年志愿者中收集刺激唾液以分离变形链球菌，并用任意引物OPA 02、03、05、13和18对总共88株分离株进行基因分型。在唾液样本中鉴定出14种不同的基因型。大多数志愿者（11人中的9人）仅呈现一种基因型。本研究结果表明，引物OPA 02、03、05和13适用于成年志愿者唾液中变形链球菌分离株的基因型鉴定。
