Health Sciences, School of Medicine, Autonomous University of State of Mexico, Toluca City, Mexico.
J Appl Oral Sci. 2012 Sep-Oct;20(5):544-9. doi: 10.1590/s1678-77572012000500009.
In orthodontics, fixed appliances placed in the oral cavity are colonized by microorganisms.
The purpose of this study was to quantitatively determine the independent bacterial colonization of S. mutans and S. sobrinus in orthodontic composite resins.
Seven orthodontic composite adhesives for bonding brackets were selected and classified into 14 groups; (GIm, GIs) Enlight, (GIIm, GIIs) Grengloo, (GIIIm, GIIIs) Kurasper F, (GIVm, GIVs) BeautyOrtho Bond, (GVm, GVs) Transbond CC, (GVIm, GVIs) Turbo Bond II, (GVIIm, GVIIs) Blugloo. 60 blocks of 4x4x1 mm of each orthodontic composite resin were made (total 420 blocks), and gently polished with sand-paper and ultrasonically cleaned. S. mutans and S. sobrinus were independently cultivated. For the quantitative analysis, a radioactive marker was used to codify the bacteria (³H) adhered to the surface of the materials. The blocks were submerged in a solution with microorganisms previously radiolabeled and separated (210 blocks for S. mutans and 210 blocks for S. sobrinus) for 2 hours at 37 ºC. Next, the blocks were placed in a combustion system, to capture the residues and measure the radiation. The statistical analysis was calculated with the ANOVA test (Sheffè post-hoc).
Significant differences of bacterial adhesion were found amongst the groups. In the GIm and GIs the significant lowest scores for both microorganisms were shown; in contrast, the values of GVII for both bacteria were significantly the highest.
This study showed that the orthodontic composite resin evaluated in the GIm and GIs, obtained the lowest adherence of S. mutans and S. sobrinus, which may reduce the enamel demineralization and the risk of white spot lesion formation.
本研究旨在定量测定正畸复合树脂中变异链球菌和远缘链球菌的独立细菌定植。
选择 7 种用于粘接托槽的正畸复合粘接剂，并分为 14 组；（GIm、GIs）Enlight，（GIIm、GIIs）Grengloo，（GIIIm、GIIIs）Kurasper F，（GIVm、GIVs）BeautyOrtho Bond，（GVm、GVs）Transbond CC，（GVIm、GVIs）Turbo Bond II，（GVIIm、GVIIs）Blugloo。每组制作 60 个 4x4x1mm 的正畸复合树脂块（共 420 个），用砂纸轻轻打磨并用超声清洗。分别培养变异链球菌和远缘链球菌。为了定量分析，使用放射性标记物对附着在材料表面的细菌进行编码（³H）。将块浸入先前放射性标记和分离的微生物溶液中（210 块用于变异链球菌和 210 块用于远缘链球菌），在 37℃下孵育 2 小时。然后，将块放入燃烧系统中，捕获残留物并测量辐射。采用方差分析（Sheffè 事后检验）进行统计分析。
发现各实验组之间的细菌黏附存在显著差异。在 GIm 和 GIs 中，两种微生物的得分均显著最低；相比之下，两种细菌的 GVII 值均显著最高。
本研究表明，评估的正畸复合树脂在 GIm 和 GIs 中获得了最低的变异链球菌和远缘链球菌黏附，这可能降低釉质脱矿和白斑形成的风险。
