Datta H K, Sullivan M, Rathod H, MacIntyre I
Department of Chemical Pathology, Royal Postgraduate Medical School, London, U.K.
Biochem Biophys Res Commun. 1991 Aug 15;178(3):940-5. doi: 10.1016/0006-291x(91)90982-d.
The metabolites of cycloxyegenase and lipoxygenase pathways are known to play an important role in the bone metabolism involving osteoclast and osteoblast interaction, bone resorption and morphogenesis. The recently discovered growth factor TGF-beta is abundant in bone and some of its intracellular and extracellular effects depend on de novo synthesis of eicosanoids. However, the effect of TGF-beta on the synthesis and the release of eicosanoid by bone cells is essentially unknown. In the present study we have identified the main eicosanoid metabolites produced by osteogenic osteosarcoma cell-line SAOS1 and investigated how production and release of these is affected by TGF-beta. We found that the leukotriene C4 is the main metabolite produced by these cells and that TGF-beta induces concentration-dependent, quantitative and qualitative alterations in eicosanoid production and release by human osteogenic osteosarcoma cells SAOS1.
环氧合酶和脂氧合酶途径的代谢产物在涉及破骨细胞和成骨细胞相互作用、骨吸收和形态发生的骨代谢中起着重要作用。最近发现的生长因子转化生长因子-β（TGF-β）在骨中含量丰富，其一些细胞内和细胞外效应依赖于类花生酸的从头合成。然而，TGF-β对骨细胞类花生酸合成和释放的影响基本上尚不清楚。在本研究中，我们确定了成骨性骨肉瘤细胞系SAOS1产生的主要类花生酸代谢产物，并研究了TGF-β如何影响这些产物的产生和释放。我们发现白三烯C4是这些细胞产生的主要代谢产物，并且TGF-β可诱导人成骨性骨肉瘤细胞SAOS1的类花生酸产生和释放发生浓度依赖性、定量和定性改变。
