Yao Yang, Dong Yang, Lin Feng, Zhao Hui, Shen Zan, Chen Ping, Sun Yuan-Jue, Tang Li-Na, Zheng Shui-Er
Department of Oncology, Affiliated 6th People's Hospital, Shanghai Jiaotong University, Shanghai 200223, P.R. China.
Oncol Rep. 2009 Jan;21(1):229-35.
The nuclear export protein chromosomal region maintenance/exportin 1/Xpo1 (CRM1) is involved in the nuclear export of proteins and messenger RNAs and, thus, mediates the subcellular distribution of important molecules. Osteosarcoma is a ubiquitous and highly aggressive malignant bone tumor. The expression of CRM1 protein in human osteosarcoma has not been reported to date. We investigated the expression of CRM1 in 57 human osteosarcoma and 5 normal cartilage tissues. Western blot investigation revealed expression of CRM1 was significantly increased in osteosarcoma compared with normal tissues. High expression of CRM1 was significantly associated with increased serum level of alkaline phosphatase (ALP, P=0.001) but did not associate with that of lactate dehydrogenase (LDH, P=0.06). In univariate analysis, a significant association between CRM1 expression and tumor size (P=0.014) as well as histological grade (P=0.003) was observed, while high CRM1 expression was not correlated with the other clinicopathological parameters. In Kaplan-Meier survival analysis, high CRM1 expression was a significant prognostic indicator for progression-free survival (P=0.016) as well as overall survival (P=0.008). Multivariate analysis demonstrated that expression of CRM1 was an independent prognostic parameter for longer overall survival (95% CI, 1.27-5.39). Additional prospective studies are required to investigate the prognostic role of high expression of CRM1.
核输出蛋白染色体区域维持蛋白/输出蛋白1/Xpo1（CRM1）参与蛋白质和信使核糖核酸的核输出，因此介导重要分子的亚细胞分布。骨肉瘤是一种常见且侵袭性很强的恶性骨肿瘤。迄今为止，尚未有关于CRM1蛋白在人类骨肉瘤中表达的报道。我们研究了57例人类骨肉瘤组织和5例正常软骨组织中CRM1的表达情况。蛋白质印迹分析显示，与正常组织相比，骨肉瘤中CRM1的表达显著增加。CRM1的高表达与血清碱性磷酸酶（ALP）水平升高显著相关（P=0.001），但与乳酸脱氢酶（LDH）水平无关（P=0.06）。单因素分析显示，CRM1表达与肿瘤大小（P=0.014）以及组织学分级（P=0.003）之间存在显著关联，而CRM1高表达与其他临床病理参数无关。在Kaplan-Meier生存分析中，CRM1高表达是无进展生存期（P=0.016）和总生存期（P=0.008）的显著预后指标。多因素分析表明，CRM1表达是总生存期延长的独立预后参数（95%CI，1.27 - 5.39）。需要进一步的前瞻性研究来探讨CRM1高表达的预后作用。
