噬菌体蛋白质组学：质谱分析的应用

Phage proteomics: applications of mass spectrometry.

作者信息

Lavigne Rob, Ceyssens Pieter-Jan, Robben J

机构信息

Department of Biosystems, Division of Gene Technology, Katholieke Universiteit Leuven, Leuven, Belgium.

出版信息

Methods Mol Biol. 2009;502:239-51. doi: 10.1007/978-1-60327-565-1_14.

DOI:10.1007/978-1-60327-565-1_14

PMID:19082560

Abstract

Current techniques in mass spectrometry (MS) allow sensitive and accurate identification of proteins thanks to the in silico availability of these protein sequences within databases. This chapter provides a short overview of MS techniques used in the identification of phage structural proteins and focuses on an electron spray peptide ionization (ESI-MS/MS) approach to identify the phage structural proteome in a comprehensive and systematic ways. Such analyses provide an experimental examination of structural proteins and confirm genome-based gene predictions.

摘要

由于数据库中这些蛋白质序列的计算机可用性，质谱（MS）的当前技术能够灵敏且准确地鉴定蛋白质。本章简要概述了用于鉴定噬菌体结构蛋白的质谱技术，并着重介绍了一种通过电喷雾肽电离（ESI-MS/MS）方法全面系统地鉴定噬菌体结构蛋白质组的方式。此类分析为结构蛋白提供了实验性检验，并证实了基于基因组的基因预测。