Steiner Barbara, Kurz Harald, Lemmens Marc, Buerstmayr Hermann
University of Natural Resources and Applied Life Sciences, Vienna, Austria.
Theor Appl Genet. 2009 Feb;118(4):753-64. doi: 10.1007/s00122-008-0935-8. Epub 2008 Dec 10.
Fusarium head blight (FHB) is a devastating disease of wheat. Molecular mapping led to the identification of two major FHB resistance QTL, Fhb1 and Qfhs.ifa-5A. The actual function of these resistance genes is still unknown. The resistant line CM82036, the susceptible line Remus and two sister lines from the cross CM82036/Remus were analysed for gene expression. The sister lines show contrasting levels of FHB resistance due to the presence or absence of resistance alleles at Fhb1 and Qfhs.ifa-5A. At anthesis plants were challenged by Fusarium graminearum or water under controlled conditions. At six-time points after inoculation (0-72 h) gene expression of specific wheat floral tissue was analysed by cDNA-AFLPs in two biological replications. Altered expression patterns after F. graminearum inoculation were observed for 164 transcript-derived fragments (TDFs), corresponding to 3.4% of the analysed fragments. Fourteen TDFs, 0.28% of the total analysed fragments, displayed differential expression after fungal attack depending on the genotype; five of these TDFs were differentially expressed between the sister lines and are possibly associated with the possession of Fhb1 and Qfhs-ifa-5A and the FHB resistance level of the genotypes. Sequencing and annotation of these gene tags revealed homologies to a UDP-glucosyltransferase, phenylalanine ammonia-lyase, Dna-J like protein, pathogenesis-related family protein and to one gene with unknown function providing initial clues for guiding further functional studies on the resistance reaction of wheat against FHB. This work is the first report on differential gene expression between related, resistant and susceptible, wheat lines after F. graminearum attack.
小麦赤霉病（FHB）是小麦的一种毁灭性病害。分子图谱分析鉴定出了两个主要的小麦赤霉病抗性数量性状位点（QTL），即Fhb1和Qfhs.ifa - 5A。这些抗性基因的实际功能仍然未知。对抗性品系CM82036、感病品系雷穆斯以及CM82036/雷穆斯杂交后代的两个姊妹系进行了基因表达分析。由于Fhb1和Qfhs.ifa - 5A抗性等位基因的存在与否，姊妹系表现出不同水平的小麦赤霉病抗性。在花期，将植株在可控条件下接种禾谷镰刀菌或用水处理。接种后六个时间点（0 - 72小时），通过cDNA - AFLP技术在两个生物学重复中分析特定小麦花组织的基因表达。在接种禾谷镰刀菌后，观察到164个转录本衍生片段（TDF）的表达模式发生改变，占分析片段的3.4%。其中14个TDF（占总分析片段的0.28%）在真菌侵染后根据基因型表现出差异表达；其中5个TDF在姊妹系之间差异表达，可能与Fhb1和Qfhs - ifa - 5A的存在以及基因型的小麦赤霉病抗性水平有关。对这些基因标签进行测序和注释后发现，它们与一个UDP - 葡萄糖基转移酶、苯丙氨酸解氨酶、Dna - J样蛋白、病程相关家族蛋白以及一个功能未知的基因具有同源性，为指导进一步研究小麦对小麦赤霉病抗性反应的功能研究提供了初步线索。这项工作是关于禾谷镰刀菌侵染后相关的抗性和感病小麦品系之间差异基因表达的首次报道。
