Bernardo Amy, Bai Guihua, Guo Peiguo, Xiao Kai, Guenzi Arron C, Ayoubi Patricia
Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA.
Funct Integr Genomics. 2007 Jan;7(1):69-77. doi: 10.1007/s10142-006-0028-1. Epub 2006 Apr 25.
Fusarium head blight (FHB), primarily caused by Fusarium graminearum Schw., is a destructive disease of wheat (Triticum aestivum L.). Although several genes related to FHB resistance have been reported, global analysis of gene expression in response to FHB infection remains to be explored. The expression patterns of transcriptomes from wheat spikes of FHB-resistant cultivar Ning 7840 and susceptible cultivar Clark were monitored during a period of 72 h after inoculation (hai) with F. graminearum. Microarray analysis, coupled with suppression subtractive hybridization technique, identified 44 significantly differentially expressed genes between cv. Ning 7840 and cv. Clark. More differentially expressed genes were identified from susceptible libraries than from resistance libraries. The up-regulation of defense-related genes in Ning 7840 relative to cultivar Clark occurred during early fungal stress (3-12 hai). Three genes, with unknown function that were up-regulated in cv. Ning 7840 at most time points investigated, might play an important role in enhancing FHB resistance.
小麦赤霉病（FHB）主要由禾谷镰刀菌（Fusarium graminearum Schw.）引起，是小麦（Triticum aestivum L.）的一种毁灭性病害。尽管已经报道了几个与抗FHB相关的基因，但对FHB感染响应的基因表达的全局分析仍有待探索。在接种禾谷镰刀菌后72小时内，监测了抗FHB品种宁7840和感病品种Clark的小麦穗转录组的表达模式。微阵列分析结合抑制性消减杂交技术，在宁7840和Clark品种之间鉴定出44个显著差异表达的基因。从感病文库中鉴定出的差异表达基因比抗病文库中的更多。相对于Clark品种，宁7840中防御相关基因的上调发生在早期真菌胁迫期间（接种后3 - 12小时）。在大多数研究时间点，宁7840中三个功能未知的基因上调，可能在增强对FHB的抗性中起重要作用。
