Szathmáry Erzsébet, Nádudvari Júlia Novák, Szabó László, Tóbiás István, Balázs Ervin, Palkovics László
Department of Plant Pathology, Faculty of Horticultural Science, Corvinus University of Budapest, Budapest, Hungary.
Arch Virol. 2009;154(1):141-5. doi: 10.1007/s00705-008-0281-9. Epub 2008 Dec 11.
Plum pox virus (PPV) isolates were collected in Hungary from plum varieties. PCR targeting the 3' genomic region resulted in a shorter PCR product in the case of the B1298 isolate bearing a 135-nucleotide deletion in frame in the N-terminal part of the coat protein (CP). The isolate was aphid-transmissible and the virion diameter was reduced compared to PPV-SK68. Detectability of this isolate by Western blot varied according to the antibody used. Integration of the deleted CP gene into an infectious PPV clone had no effect on infectivity and symptomatology. In competition experiments, B1298 had a considerable advantage in virus accumulation.
李痘病毒（PPV）分离株是从匈牙利的李品种中收集的。针对3'基因组区域进行PCR时，对于在外壳蛋白（CP）N端部分存在135个核苷酸框内缺失的B1298分离株，得到的PCR产物较短。该分离株可通过蚜虫传播，与PPV-SK68相比，病毒粒子直径减小。通过蛋白质免疫印迹法检测该分离株时，其可检测性因所用抗体而异。将缺失的CP基因整合到感染性PPV克隆中对感染性和症状没有影响。在竞争实验中，B1298在病毒积累方面具有相当大的优势。
