Estes Matthew D, Do Jaephil, Ahn Chong H
Department of Biomedical Engineering, MicroSystems and BioMEMS Lab, University of Cincinnati, 814 Rhodes Hall, Cincinnati, OH 45221, USA.
Biomed Microdevices. 2009 Apr;11(2):509-15. doi: 10.1007/s10544-008-9257-5.
This paper presents an on-chip magnetic cell sorting system for the sorting of cells based on a variety of surface markers. A polymer lab on a chip integrated with an electroplated array of Ni/Fe permalloy has been designed, fabricated, and characterized for the separation of cell substitutes at a variety of flow rates and incubation times. The system sequentially labels cell substitutes with magnetic beads and sorts them, repeating this process to sort for a variety of surface markers. Flow rates and incubation times were varied to characterize the system and produce the best combination of high specific capture and low nonspecific capture. The separation system developed on polymer is selective and efficient while being low cost, portable, and fabricated in a modular structure that can be integrated with other cell handling processes.
本文介绍了一种基于多种表面标志物进行细胞分选的片上磁细胞分选系统。设计、制造并表征了一种集成有镍铁坡莫合金电镀阵列的聚合物芯片实验室，用于在各种流速和孵育时间下分离细胞替代物。该系统依次用磁珠标记细胞替代物并进行分选，重复此过程以分选多种表面标志物。改变流速和孵育时间以表征该系统，并产生高特异性捕获和低非特异性捕获的最佳组合。在聚合物上开发的分离系统具有选择性和高效性，同时成本低、便于携带，并且以模块化结构制造，可以与其他细胞处理过程集成。
