Combining multiple optical trapping with microflow manipulation for the rapid bioanalytics on microparticles in a chip.

作者信息

Boer G, Johann R, Rohner J, Merenda F, Delacrétaz G, Renaud Ph, Salathé R-P

机构信息

ARCoptix S.A., Ch. Trois-Portes 18, NE-2000 Neuchâtel, Switzerland.

出版信息

Rev Sci Instrum. 2007 Nov;78(11):116101. doi: 10.1063/1.2804768.

Abstract

An array of four independent laser traps is combined with a polydimethylsiloxane microfluidic chip to form a very compact system allowing parallel processing of biological objects. Strong three dimensional trapping allows holding objects such as functionalized beads in flows at speeds near 1 mm/s, enabling rapid processing. By pressure control of the inlet flows, the trapped objects can be put in contact with different solutions for analysis purpose. This setup, including a fluorescence excitation-detection scheme, offers the potential to perform complex biochemical manipulations on an ensemble of microparticles.

摘要

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