Laboratoire de Biochimie et de Toxicologie Environnementale, Institut Supérieur d'Agronomie de Chott-Mariem, 4042 Sousse, Tunisia.
Fish Physiol Biochem. 2010 Mar;36(1):101-7. doi: 10.1007/s10695-008-9295-1. Epub 2008 Dec 11.
The levels of metallothionein (MT), a biomarker of metal exposure, and of Cd and Cu, known as MT inducers, were investigated in Sparus aurata intraperitoneally injected with 500 microg/kg of Cu and Cd for 2 days. MT and metal concentrations (Cd and Cu) were determined in liver, gills and kidney. MT levels were significantly increased in all investigated tissues, with the highest value in liver of Cu as Cd-treated fishes (3.56-fold and 3.3- fold, respectively). Metal concentrations were statistically different between all tissues. Highest metal concentrations were in the liver. The higher metal concentrations and MT induction levels support the main role of MT in metal homeostasis and detoxification.
研究人员将 500 微克/千克的铜和镉通过腹腔注射的方式注入到真鲷体内，持续两天后，检测了其金属硫蛋白（MT）的水平。MT 是金属暴露的生物标志物，而铜和镉则是众所周知的 MT 诱导剂。研究人员还测定了肝脏、鳃和肾脏中的 MT 和金属浓度（镉和铜）。结果表明，所有检测组织中的 MT 水平都显著增加，其中以肝脏中的铜处理鱼类的 MT 水平增加最多（分别为 3.56 倍和 3.3-3.56 倍）。所有组织之间的金属浓度也存在统计学差异。肝脏中的金属浓度最高。较高的金属浓度和 MT 诱导水平支持 MT 在金属稳态和解毒中的主要作用。
