Laboratoire de Biochimie et de Toxicologie Environnementale, Ecole Supérieure d'Horticulture et d'Elevage, Chott-Marièm, 4042, Tunisia.
Fish Physiol Biochem. 2006 Mar;32(1):93-8. doi: 10.1007/s10695-006-0041-2.
The potential use of acetylcholinesterase (AChE) and metallothionein (MT) responses as biomarker of organophosphorous (OPs) and trace metal were assessed in fish Seriola dumerilli exposed to 0, 4, 6 mg/kg of malathion for 2, 7 and 13 days, and to 0, 50, 100, 250 mug/kg of Cd for 2 days. Brain AChE was significantly inhibited after 2 and 7 days of malathion exposure, in a dose-response manner, but no inhibition was observed after 13 days of exposure. When exposed to Cd for 2 days, S. dumerelli presented an increase in AChE activity at a concentration of 50 mug/kg, but a strong and dose-dependent AChE inhibition at 100 and 250 mug/kg. Cd treatment also caused a rapid increase in MTs concentration in liver, even at the lower concentration. Our experiments indicate that the measurement of hepatic MT concentration and brain AChE activity in S. dumerilli would be useful biomarkers of OP and Cd exposure and/or effects.
评估了乙酰胆碱酯酶（AChE）和金属硫蛋白（MT）的潜在作用，作为暴露于马拉硫磷（0、4、6mg/kg）2、7 和 13 天和镉（0、50、100、250μg/kg）2 天的达氏鲷鱼的器官磷（OPs）和痕量金属的生物标志物。脑 AChE 在马拉硫磷暴露 2 和 7 天后呈剂量反应方式显著抑制，但暴露 13 天后未见抑制。当暴露于 2 天的镉时，S. dumerelli 在 50μg/kg 的浓度下表现出 AChE 活性的增加，但在 100 和 250μg/kg 时表现出强烈且剂量依赖性的 AChE 抑制。镉处理还导致肝脏中 MTs 浓度的快速增加，即使在较低的浓度下也是如此。我们的实验表明，测量达氏鲷鱼肝脏 MT 浓度和脑 AChE 活性将是 OP 和 Cd 暴露和/或影响的有用生物标志物。
